Research Article

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Cloning; Penta D allele; Sequencing; Short tandem repeat; Zhuang nationality

Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify ... more

Y.L. Yang; J.G. Wang; D.X. Wang; W.Y. Zhang; X.J. Liu; J. Cao; S.L. Yang
Han Chinese population; Rheumatoid arthritis; Sequencing; Sialic acid acetylesterase; Single nucleotide polymorphism

The function of rare genotypes encoding defective variants of sialic acid acetylesterase (SIAE) in some autoimmune diseases, including rheumatoid arthritis (RA), is ambiguous. We determined whether mutations in the SIAE gene are responsible for RA in a Han Chinese population.DNA was prepared from the venous leukocytes of 444 RA patients and 647 normal controls. The coding regions ... more

D.D. Zhang; F. He; H.T. Liu; F. Hao; J. Zhu
Congenital cataract; Genes; Loci; Sequencing; Sex-linked inheritance

Congenital cataract is a common cause of blindness in children; however, its pathogenesis remains unclear. Genetic factors have been shown to play an important role in the pathogenesis of congenital cataract. The current genetic models of congenital cataract include autosomal dominant, autosomal recessive, and sex-linked inheritance. Sex-linked congenital cataract could be inherited ... more

D.D. Zhang; J.Z. Du; J. Topolewski; X.M. Wang; D.D. Zhang; J.Z. Du; J. Topolewski; X.M. Wang
Biotyping; Genetic and phylogenetic analysis; Immunocapture RT-PCR; PVYNTN; Sequencing; Serotyping

In Tunisia, potato virus Y (PVY) currently presents a significant threat to potato production, reducing tuber yield and quality. Three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in Tunisia were tested for PVY infection by DAS-ELISA. The virus was detected in all regions ... more

M. Tayahi; C. Gharsallah; N. Khamassy; H. Fakhfakh; F. Djilani-Khouadja; M. Tayahi; C. Gharsallah; N. Khamassy; H. Fakhfakh; F. Djilani-Khouadja
Lactation; PCR; Serology; Sheep; Toxoplasmosis

The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

deSantana Rocha; R.Lde Sousa Moura; B.M. Maciel; L.A. Guimarães; H.N.S. O’dwyer; A.D. Munhoz; G.R. Albuquerque
Genetic relationship; PCR; Polymorphism; RAPD

The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke hybrid lines. The degree ... more

M.A. Sharaf-Eldin; A. Al-Tamimi; P. Alam; S.F. Elkholy; J.R. Jordan
Forestry species; Genetic parameters; PCR

Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of which 98.99% were ... more

L.S. Costa; L.R.S. Reiniger; B.M. Heinzmann; L.P. Amaral; C.M.L. Serrote
Fragile X syndrome; Intellectual disability; PCR; PCR-P; PCR-T

The aim of this study was to validate the molecular genetic diagnosis of patients suspected of Fragile X Syndrome (FXS) in the Laboratory of Human Cytogenetics and Molecular Genetics (LaGene) of the Department of Health of the State of Goiás, using polymerase chain reaction (PCR). Thirty-five patients referred by public health doctors to LaGene, indicating clinical diagnosis of FXS, were ... more

A.P. Amancio; C.Ade O. Melo; deM. Vieira; L.B. Minasi; deM. e Silva; C.C. da Silva; A.D. da Cruz
High-throughput; Homologous recombination; In vivo cloning; PCR

In this study, we optimized a restriction-ligation-free (RLF) method to save time and cost of constructing multiple plasmids with the same gene insert, and examined the efficacy of RLF on high-throughput multi-plasmid cloning. This method utilizes the precise DNA repair and recombination systems within Escherichia coli, which allows to bypass the in vitro restriction and ... more

Y. Wang; Y. Liu; J. Chen; M.J. Tang; S.L. Zhang; L.N. Wei; C.H. Li; D.B. Wei
Correlation; miR-21; Multiple myeloma; PCR

The aim of the present study is to examine the expression level of peripheral mir-21 in multiple myeloma (MM) patients and to determine its clinical significance. MM patients (30), monoclonal gammopathy of undetermined significance (MGUS) patients (14), and normal controls (20) were recruited to determine the serum level of β2-MG, IgA and IgM, IgG, λ, κ, TP, ALB, Hb, LDH, and Ca2+ ... more

J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang; J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang; J.H. Wang; W.W. Zhou; B.X. Liu; D.L. Man; Z.D. Yang; F.R. Liu; H. Shang