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The objective of this study was to verify whether Toxoplasma gondii is excreted in the milk of naturally infected sheep. In order to accomplish this, 275 lactating ewes were used; these were bred extensively in 17 estates distributed across nine cities. Polymerase chain reaction amplification was used to detect T. gondii DNA in milk samples, and the indirect ... more

deSantana Rocha; R.Lde Sousa Moura; B.M. Maciel; L.A. Guimarães; H.N.S. O’dwyer; A.D. Munhoz; G.R. Albuquerque
Different tail type; FAS; HSL; mRNA and protein expression; PPARγ; Sheep

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan sheep (short fat tail), Kazakh sheep (hip fat tail), and Tibetan sheep ( ... more

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Antimicrobial peptides; Innate immunity; Sheep; Sheep beta defensin-2; Single nucleotide polymorphism

The northern region of Brazil produces a large number of sheep, with Pará being the largest sheep breeding state in the region. In the Amazon region, livestock production is a challenge due to the high diversity of pathogens affecting humans and animals. Defensins are antimicrobial peptides acting as a first barrier against micro-organisms and present high variation in different organisms ... more

B.B. Souza; E.M. Barbosa; J.S.N. Azevedo; J.E.G. Campelo; L.F.S. Rodrigues; L.M.L. Pinheiro; S.C.B. Silva; A.S. Schierholt; P.H. Souza; E.C. Gonçalves; S. Filho
Liver tissue; RNA-seq; Sheep; Transcriptome

Research on gene regulation has been made possible with the help of RNA sequencing applications such as RNA-Seq technology for high-throughput sequencing platforms. Recent studies have explored the transcriptomes from different tissues of domestic animals using RNA-Seq technology, but little research has been done to study the transcriptomes of breeds of sheep having different adipose ... more

X. Cheng; S.G. Zhao; Y. Yue; Z. Liu; H.W. Li; J.P. Wu; X. Cheng; S.G. Zhao; Y. Yue; Z. Liu; H.W. Li; J.P. Wu
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Animals; Apoptosis; Cells, Cultured; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Gonadotropins, Equine; Horses; In Vitro Oocyte Maturation Techniques; Oocytes; Receptors, FSH; Receptors, LH; Receptors, LHRH; Sheep

We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM ... more

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Animals; Gene expression profiling; Gene Expression Regulation; Genetic markers; Oligonucleotide Array Sequence Analysis; Phenotype; Quantitative trait loci; Sheep; Wool

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provide a molecular basis for improving wool quality in sheep. Genes ... more

Y.Z. Tian; T. Usman; K.C. Tian; J. Di; X.X. Huang; X.M. Xu; H. Tulafu; W.W. Wu; X.F. Fu; Y. Bai; M. Tuerxun; A. Maimaiti
Biometric; Selection; SNPs; Statistics

The aim of this study was to evaluate different methods used in genomic selection, and to verify those that select a higher proportion of individuals with superior genotypes. Thus, F2 populations of different sizes were simulated (100, 200, 500, and 1000 individuals) with 10 replications each. These consisted of 10 linkage groups (LG) of 100 cM each, containing 100 equally spaced markers ... more

L.L. Bhering; V.S. Junqueira; L.A. Peixoto; C.D. Cruz; B.G. Laviola
Idiopathic azoospermia; Idiopathic oligospermia; Pygo2 gene; SNPs

Male infertility is often associated with a decreased sperm count. The Pygo2 gene is expressed in the elongating spermatid during chromatin remodeling; thus impairment in PYGO2 function might lead to spermatogenic arrest, sperm count reduction, and subsequent infertility. The aim of this study was to identify mutations in Pygo2 that might lead to idiopathic oligospermia ... more

S.Q. Ge; J. Grifin; L.H. Liu; K.I. Aston; L. Simon; T.G. Jenkins; B.R. Emery; D.T. Carrell
Body size; Haplotype; IGFBP-6; Mini-pig; SNPs

Insulin-like growth factor binding protein-6 (IGFBP-6) is a member of the IGFBP family, which is known to be a key factor in regulating the effect of insulin-like growth factor-2 (IGF-2) on the animal growth and development. Gene sequences of 3'-untranslated regions (UTR) and exon 4 of IGFBP-6 may influence the expression and proteolysis of IGFBP-6. In this study, 551 bp of the ... more

X.B. Fang; S.C. Liu; Q.Y. Wu; S.M. Li; Y.Y. Cheng; H.Y. Fu; C. Lu; D. Su; H. Yu; L.L. Hao
APM1; Disorders of lipid metabolism; HDCP; SNPs

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) in APM1 contribute to disorders of lipid metabolism in hypertensive disorder complicating pregnancy (HDCP). The study included 178 pregnant women with HDCP and 243 healthy pregnant controls. Using PCR-restriction fragment length polymorphism, we detected the frequencies of genotypes, alleles, ... more

Y. Wang; R.X. Liu; H. Liu