Research Article

Related GMR Articles

09/13/2012
Aptamer; Asthma; Nanotechnology; pRNA; siRNA; STAT5b

Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to their successful ... more

C. Qiu; W.K. Peng; F. Shi; T. Zhang
09/22/2015
Bombyx mori; marker-assisted selection; Scaleless wings; Tolerance to fluoride

In the silkworm (Bombyx mori), tolerance to fluoride and scaleless wings are controlled by the dominant gene Dtf (dominant tolerance to fluoride) and recessive gene nlw (no Lepidoptera wings), respectively, and these genes have been mapped by using simple sequence repeat and sequence tag site markers. Marker-assisted evaluation and selection of silkworms with ... more

M.W. Li; H.J. Yu; X.L. Yi; J. Li; F.Y. Dai; C.X. Hou
08/29/2016
dCAPS; marker-assisted selection; Purity detection; tms5 gene; Two-line rice

Molecular markers can increase both the efficiency and speed of breeding programs. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. In this study, we used newly derived cleaved amplified polymorphic sequence markers to detect the functional mutations of tms5, which is a male sterile gene that is ... more

F.S. Song; J.L. Ni; Y.L. Qian; L. Li; D.H. Ni; J.B. Yang; F.S. Song; J.L. Ni; Y.L. Qian; L. Li; D.H. Ni; J.B. Yang
12/01/2015
Bladder cancer; Gene polymorphism; PCR-RFLP; Toll-like receptor

In this study, the association between the 729G/C polymorphism in Toll-like receptor 4 (TLR4) and the risk of bladder cancer was investigated. A total of 376 patients with bladder cancer and 380 healthy volunteers from the Third Xiangya Hospital of Central South University (China) were enrolled in this study between January 2008 and February 2014. The TLR4-729G/C polymorphism was ... more

B. Wan; J. Tan; Q. Zeng; L.Y. He; Y. Gan; Y.B. Dai; K. Yao
10/19/2015
Coat color; meat quality; Native pig breeds; PCR-RFLP; Vertebra number

Mutations in melanocortin 1 receptor (MC1R) gene and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) gene have been shown to affect coat color patterns in pigs. Additional functional marker genes, such as insulin like growth factor-2 (IGF2) and orphan nuclear receptor, germ cell nuclear factor (NR6A1), have been described for ... more

P. Klomtong; K. Chaweewan; Y. Phasuk; M. Duangjinda
03/11/2016
Goat; PCR-RFLP; Polymorphism; POU1F1 gene

As a member of the POU-domain family, the POU1F1 is a positive regulator for growth hormone, prolactin and thyroid-stimulating hormone b, by binding to target DNA promoters as a dimer in mammals. This study described the polymorphisms at the goat POU1F1-DdeI locus and analyzed the distribution of alleles in 15 indigenous Chinese goat breeds. The PCR-RFLP analysis showed ... more

M.J. Li; C.M. Zhang; X.Y. Lan; X.T. Fang; C.Z. Lei; H. Chen; M.J. Li; C.M. Zhang; X.Y. Lan; X.T. Fang; C.Z. Lei; H. Chen
01/12/2018
Biological nitrogen fixation; Bradyrhizobium; Inoculation; PCR-RFLP; Soybean

Soybean (Glycine max) is considered one of the most economically important agricultural crops in Brazil. Due to the high protein content of its grains, this crop demands a greater amount of nitrogen (N) for production. In general, for soybean, nitrogen fertilization costs through chemical fertilizers are considered high. However, the biological nitrogen fixation ( ... more

J.G. Perin; M.B.D. Bertéli; J.S. do Valle; G.A. Linde; L.D.Paccola Meirelles; N.B. Colauto; F.G. Barcellos
03/26/2020
ERIC-PCR; MALDI-TOF; Molecular phylogeny; PCR-RFLP; Yeast identification

Pigeon droppings carry microorganisms such as Candida spp., which have high genetic variability and are the most common opportunistic pathogens in humans. There is a need to establish fast, reliable and simple laboratory methods to differentiate pathogenic from non-pathogenic yeast species in pigeon droppings, as they can be a source of contamination for ... more

J. Santos; M.A.S. Xavier; L. Cardoso; S.A.M. Nobre; R.R. Bacchi; C.H.C. Cangussu; A.C. Almeida; L.N. Leite; N.A.P. Barreto; A.R.E.O. Xavier