Research Article

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Alpha 1-antitrypsin; Alpha-fetoprotein; Proteins; Transferring; Western blotting; Yolk sac

The yolk sac is an embryonic membrane that is essential for the embryo’s initial survival in many mammals. It also plays an important role in the production of proteins necessary for development. We studied proteins of the yolk sac in bovine embryos at up to 40 days of gestation. We examined the yolk sac of 17 bovine embryos at different gestational periods, measuring α-fetoprotein, α-1- ... more

F.S. Matsumoto; V.C. Oliveira; C.A.F. Mançanares; C.E. Ambrósio; M.A. Miglino
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Apoptosis; Fetal membranes; Metabolic pathways; Metabolites; Proteins

In several species, placentation involves the presence of two different membranes responsible for maternal-fetal exchanges: the yolk sac and the chorioallantoic placenta. The yolk sac plays important roles in embryonic survival, mainly during the early stages of gestation. In bovine, it is a transitional membrane that is present until day 50-70 of pregnancy. Herein, we evaluated the ... more

A.C. Galdos-Riveros; P.O. Favaron; S.E.A.L. Will; M.A. Miglino; D.A. Maria
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Female; Genetic predisposition to disease; High-Throughput Nucleotide Sequencing; Humans; Macular Degeneration; male; Proteins; Risk; Turkey

Age-related macular degeneration (AMD) is the leading cause of blindness in developed countries. It is a complex disease with both genetic and environmental risk factors. To improve clinical management of this condition, it is important to develop risk assessment and prevention strategies for environmental influences, and establish a more effective treatment approach. The aim of the present ... more

H. Bardak; M. Gunay; Y. Ercalik; Y. Bardak; H. Ozbas; O. Bagci
02/23/2017
Algorithms; Amino acid sequence; Amino acids; Animals; binding sites; Computational biology; Databases, Protein; Humans; Hydrophobic and Hydrophilic Interactions; Models, Theoretical; Neural Networks (Computer); Phosphorylation; Proteins; Reproducibility of Results; Support vector machine

Phosphorylation is an important part of post-translational modifications of proteins, and is essential for many biological activities. Phosphorylation and dephosphorylation can regulate signal transduction, gene expression, and cell cycle regulation in many cellular processes. Phosphorylation is extremely important for both basic research and drug discovery to rapidly and correctly identify ... more

R.Z. Han; D. Wang; Y.H. Chen; L.K. Dong; Y.L. Fan
03/15/2017
Amino acid sequence; Base Sequence; Computational biology; Databases, Genetic; Humans; Models, Molecular; Open Reading Frames; Precision Medicine; Proteins; Sequence alignment; Sequence Analysis, DNA

Technological advancements in recent years have promoted a marked progress in understanding the genetic basis of phenotypes. In line with these advances, genomics has changed the paradigm of biological questions in full genome-wide scale (genome-wide), revealing an explosion of data and opening up many possibilities. On the other hand, the vast amount of information that has been generated ... more

W.J.S. Diniz; F. Canduri
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Bactrocera dorsalis; Insecticide resistance; Mass spectrometry; Trichlorphon; Two-dimensional gel electrophoresis

Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-treatment ... more

T. Jin; L. Zeng; Y.Y. Lin; Y.Y. Lu; G.W. Liang
05/13/2016
Mass spectrometry; Mycelium; Nitrogen stress; Proteome; Rice blast fungus

Magnaporthe oryzae is an important model system in studies of plant pathogenic fungi, and nitrogen is a key nutrient source affecting microbial growth and development. In order to understand how nitrogen stress causes changes in mycelial proteins, we analyzed differentially expressed mycelial proteins from the M. oryzae virulent strain CH-63 using two-dimensional ... more

X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao; X.G. Zhou; P. Yu; C. Dong; C.X. Yao; Y.M. Ding; N. Tao; Z.W. Zhao
10/19/2012
Bulk segregant analysis; genetic mapping; Vigna unguiculata

AFLP markers combined with the bulk segregant analysis methodology was used for the identification of molecular markers associated with the cowpea golden mosaic virus (CGMV) resistance gene in 286 F2 cowpea plants derived from the cross IT97K-499-35 x Canapu T16. Segregation data in the F2 population demonstrated that tolerance to CGMV is controlled by a single ... more

M.A. Rodrigues; C.A.F. Santos; J.R.F. Santana
02/08/2012
Cowpea; Genetic diversity; Germplasm management; Microsatellites; Vigna unguiculata

Genetic diversity and phylogenetic relationships among 22 local cowpea (Vigna unguiculata) varieties and inbred lines collected throughout Senegal were evaluated using simple sequence repeat molecular markers. A set of 49 primer combinations were developed from cowpea genomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea ... more

F.A. Badiane; B.S. Gowda; N. Cissé; D. Diouf; O. Sadio; M.P. Timko
03/31/2014
Callosobruchus maculates; Inter simple sequence repeat; Vigna unguiculata

The cowpea weevil (Callosobruchus maculatus Fabr.) is the most destructive pest of the cowpea bean; it reduces seed quality. To control this pest, resistance testing combined with genetic analysis using molecular markers has been widely applied in research. Among the markers that show reliable results, the inter-simple sequence repeats (ISSRs) (microsatellites) are noteworthy. ... more

M.X.Vila Nova; N.G.A. Leite; L.M. Houllou; L.V. Medeiros; A.C.Lira Neto; B.S. Hsie; L.R. Borges-Paluch; B.S. Santos; C.S.F. Araujo; A.A. Rocha; A.F. Costa

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