Research Article

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Cassava (Manihot esculenta) is a traditional crop in tropical and subtropical regions that is used for human consumption and in animal feed. This studied cassava landraces cultivated in northern Mato Grosso State, Brazil, to detect the variability in family farms, aiming at the preservation and use potential of these genetic resources. A total of 120 M ... more

E.C.M. Pedri; E.S.S. Hoogerheide; A.V. Tiago; E.S. Cardoso; J.M.A. Pinto; L.L. Santos; O.M. Yamashita; A.A.B. Rossi
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Physical localization of molecular markers and assign­ment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) were achieved using primed in situ labeling. Amplified signals for both the EST507-1 and SSRY13-5 markers were consistently observed in different stages of cell division. A comparison of the length, arm ratio, and ... more

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The population of Pará (a state in Brazil) has a very characteristic food culture, as a majority of the carbohydrates consumed are obtained from cassava (Manihot esculenta Crantz) derivatives. Tucupi is the boiled juice of cassava roots that plays a major role in the culinary footprint of Pará. Before boiling, this juice is known as manipueira and contains linamarin, a toxic ... more

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Brazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular ... more

A.V. Tiago; A.A.B. Rossi; P.V. Tiago; A.A. Carpejani; B.M. Silva; E.S.S. Hoogerheide; O.M. Yamashita; A.V. Tiago; A.A.B. Rossi; P.V. Tiago; A.A. Carpejani; B.M. Silva; E.S.S. Hoogerheide; O.M. Yamashita
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Among the factors affecting cassava yield, the leaf nutrients content has received little attention since cassava genotypes may grow well under inappropriate nutrient supply, however showing inadequate nutritional quality of tuberous roots. Considering this aspect, the genetic diversity should be explored in order to determine standard leaf nutrients in cassava ... more

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The purpose of this study was to measure the genetic diversity of wild beach plum and cultivated species, and to determine the species relationships using SSRs markers. An analysis of genetic diversity from ten beach plum germplasms was carried out using 11 simple sequence repeat (SSR) primers selected from 35 primers to generate distinct PCR products. From this plant material, 44 allele ... more

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Pinus krempfii Lecomte (Pinaceae) is an endemic tree to Vietnam with restricted habitats at higher altitudes in the highlands. In this study, genetic variation of four populations of P. krempfii was assessed using 17 microsatellite markers (single sequence repeats). Of these 17 markers, eight were polymorphic, and among the 42 putative alleles amplified, 32 were polymorphic ( ... more

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Coilia nasus; De novo transcriptome; Illumina sequencing; SSR markers

RNA-Seq technology has been widely applied to tran­scriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence re­peats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enrichment, only a few microsatellite markers ... more

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Ammopiptanthus mongolicus; De novo transcriptome; eggNOG analysis; GO functional analysis; KEGG pathway analysis; SSR markers

Ammopiptanthus mongolicus, an evergreen broadleaf legume shrub, can survive under conditions of high and low temperature, extreme salinity, and drought. This attribute makes it an ideal model for studying mechanisms of stress tolerance in plants. However, simple sequence repeat (SSR) resources for this species are insufficient in public databases. In this study, a total of 44,959 ... more

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Microsatellite markers have been widely used in the quantification of genetic variability and for genetic breeding in Musa spp. The objective of the present study was to evaluate the discriminatory power of microsatellite markers derived from ‘Calcutta 4’ and ‘Ouro’ genomic libraries, and to analyze the genetic variability among 30 banana accessions. Thirty-eight markers were ... more

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