Research Article

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Apomixis origin; Centers of diversity; Electrophoresis; Evolution; Interspecific hybridization; Polyploidy; Unreduced gametes

About 98 species of Manihot are known. All of them are native to the New World and are concentrated in four regions in Brazil and Central America. All the Manihot species so far examined have 2n = 36 chromosomes. Interspecific hybrids between cassava and its wild relatives show relatively normal meiosis, and further generations can be obtained. Electrophoresis shows ... more

N.M.A. Nassar
Apomixis; Apospory; Heterosis; Interspecific hybridization; Triploid

Apomixis means seed formation without fertilization. In cassava (Manihot esculenta) it is an alternative to reproduction by cuttings, which normally transmits pathogens and leads to an accumulation of viral and bacterial diseases. Apomixis also assures preservation of heterosis and avoids genetic segregation. It occurs in wild relatives of cassava and has been transferred ... more

N.M.A. Nassar
Basic chromosome number; Brachiaria dictyoneura; Cell fusion; Cytokinesis; Interspecific hybridization; Microsporogenesis

Microsporogenesis was analyzed in five accessions of Brachiaria dictyoneura presenting x = 6 as the basic chromosome number. All accessions were tetraploid (2n = 4x = 24) with chromosome pairing in bi-, tri-, and quadrivalents. The recorded meiotic abnormalities were those typical of polyploids, including precocious chromosome migration to the poles, laggard chromosomes, and ... more

C. Risso-Pascotto; M.S. Pagliarini; C.B. Valle
Interspecific hybridization; Passiflora L.; Paternity test; RAPD

Several interspecific Passiflora hybrids are produced in the northern hemisphere for the ornamental plant market. In Brazil, production of passion flower hybrids is limited to the introgression of genes into the main cultivated species, yellow passion fruit, to be used as rootstocks. Confirmation of hybridization in the initial developmental stage is important for breeding ... more

L.D.H.C.S. Conceição; M.M. Souza; C.B.M. Cerqueira-Silva; R.X. Corrêa; G.O. Belo; S.F. Santos
2n gamete; Interspecific hybridization; Meiosis; Pentaploidy; Poaceae

Knowledge about the cytology and reproductive behavior of a species is indispensable for hybridization programs. This is especially true for species belonging to the genus Paspalum, among which apomixis and a wide range of ploidy levels are frequently found. Paspalum conspersum Schrad. is a robust and warm-season perennial bunchgrass native to South America. Previous ... more

L. Janke; F.H.D. Souza; J.F.M. Valls; M.S. Pagliarini
Hexaploidy; Interspecific hybridization; Meiosis; Poaceae; Polyploidy

Cytological investigation revealed complete asynapsis during microsporogenesis in 2 wild accessions of Paspalum jesuiticum collected in distinct Brazilian regions. Both accessions were hexaploid (2n = 6x = 60) and 60 univalents could be counted at diakinesis. In this phase, the majority of meiocytes exhibited univalents with both chromatids. After alignment at the metaphase plate ... more

R.A.Bernardo Filho; A.C.C. Santos; F.H.D. Souza; J.F.M. Valls; M.S. Pagliarini
Edible roots; Grafting; Manihot; Periclinal chimera

We provide the first report on the synthesis of a very productive interspecific periclinal chimera of cassava, with large and edible roots. The epidermal tissue of the chimera was formed by the cultivated species Manihot esculenta (E), and the subepidermis and internal tissue were formed by the wild species, Manihot fortalezensis (F). We used cytogenetics and ... more

N.M.A. Nassar; N. Bomfim
Cassava (Manihot esculenta); Histogen layers; Periclinal chimera; Root production

Plant interspecific periclinal chimeras are a mosaic formed by tissues from two species. They are manipulated here as an efficient plant breeding tool for cassava root yields. In this study, plants ... more

P.M. Gakpetor; H. Mohammed; D. Moreti; N.M.A. Nassar
Apomixis; Expression analysis; Malus; Somatic embryogenesis receptor-like kinase

This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The full-length sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid ... more

L.J. Zhang; W.X. Dong; S.M. Guo; Y.X. Wang; A.D. Wang; X.J. Lu
2017 Aug 17
Antioxidants; beta Carotene; Diterpenes; Flowers; Linoleic Acid; Oleanolic acid; Phytol; Plant Extracts; Plant Leaves; Solanaceae; Tocopherols

In this study, the temperature and pressure of supercritical CO extraction were evaluated to obtain oleoresin of Brunfelsia uniflora leaves and flowers. The oleoresin compounds were identified by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by three different methods. The highest oleoresin yields were 3.32% at 40°C and 200 bar for the leaves, and 1.03% at 60°C ... more

L.F. Jorge; A.B. Meniqueti; R.F. Silva; K.A. Santos; E.A. Da Silva; J.E. Gonçalves; C.M. De Rezende; N.B. Colauto; Z.C. Gazim; G.A. Linde