Research Article

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Coffea arabica; Developmental stages; qRT-PCR

Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze ... more

F.C. Santos; A.C.S. Clemente; F. Caixeta; S.D.V.F. Rosa
09/12/2013
Dwarf mutant; Genetic analysis; Gibberellic acid; SSR analysis; Wheat

A spontaneous dwarf mutant of wheat was found in an F5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and ... more

X.J. Jin; D.F. Sun; H.Y. Li; Y. Yang; G.L. Sun
12/11/2015
Aedes aegypti; Isodillapiole; qRT-PCR

The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have ... more

V.S. Lima; A.C. Pinto; M.S. Rafael
11/24/2015
cDNA clone; Indigenous Chinese goats; PCR-SSCP; qRT-PCR; RERG gene

The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations with growth traits in three varieties of ... more

M.X. Sui; H.H. Wang; Z.W. Wang
04/07/2016
AHL25; Arabidopsis thaliana; Artificial micro-RNA; AtExpA8; qRT-PCR

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis ... more

A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah; A. Ud-Din; M. Rauf; S. Ghafoor; M.N.K. Khattak; M.W. Hameed; H. Shah; S. Jan; K. Muhammad; A. Rehman; Inamullah
12/15/2017
Immune response; Malaria; Phylogenetic relationship; qRT-PCR; Resistance to insecticides
Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection
of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been
used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated
to feeding and resistance to insecticides, immune response and ... more
Ohse; K. C; Matiolli; C. C; Scarpassa; V. M; Santos; V. S; Tadei; W. P; Rafael; M. S
01/03/2018
Gene cloning; Pistillata-like; qRT-PCR; RACE kit; Sedirea japonica

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No ... more

S. Jiang; S. Niu; X. Yuan; F. Liang; S. Xu; M. Wang; B. Cui
12/17/2015
Abscisic acid; Drought tolerance; Medicago sativa L.; Molybdenum cofactor sulfurase

The stress phytohormone abscisic acid (ABA) plays pivotal roles in plants’ adaptive responses to adverse environments. Molybdenum cofactor sulfurases influence aldehyde oxidase activity and ABA biosynthesis. In this study, we isolated a novel EsMcsu1 gene encoding a molybdenum cofactor sulfurase from Eutrema salsugineum. EsMcus1 transcriptional patterns varied ... more

C. Zhou; Z.Y. Ma; L. Zhu; J.S. Guo; J. Zhu; J.F. Wang
03/04/2016
Abiotic stress; Abscisic acid; Camellia sinensis (L.); Gene expression; Polyethylene glycol; SAD

Tea (Camellia sinensis L.) is a thermophilic evergreen woody plant that has poor cold tolerance. The SAD gene plays a key role in regulating fatty acid synthesis and membrane lipid fluidity in response to temperature change. In this study, full-length SAD cDNA was cloned from tea leaves using rapid amplification of cDNA ends and polymerase chain reaction (PCR)- ... more

Z.T. Ding; J.Z. Shen; L.L. Pan; Y.U. Wang; Y.S. Li; Y. Wang; H.W. Sun; Z.T. Ding; J.Z. Shen; L.L. Pan; Y.U. Wang; Y.S. Li; Y. Wang; H.W. Sun
03/30/2017
Abscisic acid; Adaptation, Physiological; Citrus; Dioxygenases; Droughts; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Stress, Physiological; Tobacco

9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) ... more

A.M. Pedrosa; L.C. Cidade; C.P.S. Martins; A.F. Macedo; D.M. Neves; F.P. Gomes; E.I.S. Floh; M.G.C. Costa

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