Research Article

Related GMR Articles

Biochemical mechanisms; Biotic stress; Pathogenesis-related proteins; Resistance to diseases

Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of Capsicum baccatum var. ... more

L.S.A. Gonçalves; R. Rodrigues; M.S.S. Diz; R.R. Robaina; A.Tdo Amaral Júnior; A.O. Carvalho; V.M. Gomes
Abiotic stress; Biotic stress; Hormone; Rice

Plant hormones play important roles in the crosstalk between biotic and abiotic stresses in rice throughout its entire growth period. However, these interactions are not completely understood. In this study, the physiological performance of rice seedlings under a single stress and a sequential combination of various stresses (intercross stress) was determined. We found that catalase, ... more

Y.P. Zhang; Z.G. E; H. Jiang; L. Wang; J. Zhou; D.F. Zhu
Genetic diversity; Polymerase chain reaction; Random amplified polymorphic DNA; Sugarcane

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage ... more

F.A. Khan; A. Khan; F.M. Azhar; S. Rauf
RAPD; Red rot; SSR; Sugarcane; Variability

Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog ... more

M.T.H. Shahid; F.A. Khan; A. Saeed; I. Fareed
DNA marker; Fingerprinting; Genetic distance; RAPD; Sugarcane

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from ... more

S. Nawaz; F.A. Khan; S. Tabasum; M. Zakria; A. Saeed; M.Z. Iqbal
DNA marker; Fingerprinting; Genetic distance; RAPD; Sugarcane

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between ... more

S. Tabasum; F.A. Khan; S. Nawaz; M.Z. Iqbal; A. Saeed
16S rRNA; Endophytic bacteria; Microbial diversity; Sugarcane

Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified ... more

G.S. Magnani; L.M. Cruz; H. Weber; J.C. Bespalhok; E. Daros; V. Baura; M.G. Yates; R.A. Monteiro; H. Faoro; F.O. Pedrosa; E.M. Souza
Endophytic fungi; Esterases; Isoenzymes; Molecular markers; rDNA; Sugarcane

This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC, with α-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as ... more

A.C. Leme; M.R.R. Bevilaqua; S.A. Rhoden; C.A. Mangolin; M.F.P.S. Machado; J.A. Pamphile
Genetic identity; Polymerase chain reaction; Simple sequence repeat; Sugarcane

Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers were used for DNA fingerprinting and genetic diversity ... more

U. Hameed; Y.B. Pan; K. Muhammad; S. Afghan; J. Iqbal
6-Benzylaminopurine; Callus; Sugarcane

A good culture system provides considerable quantities of highly regenerable target tissues. Embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. Effective exploitation of genetic transformation requires good regeneration systems. We selected three sugarcane genotypes for the establishment and optimization of ... more

S. Ijaz; I.A. Rana; I.A. Khan; M. Saleem