Research Article

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Leucine-rich repeat-receptor-like kinases; Phalaenopsis; Protocorm-like body; SERK

Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich repeat- ... more

Y.W. Huang; Y.J. Tsai; F.C. Chen
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1-Aminocyclopropanecarboxylic acid; 2-Chloroethylphosphonic acid (ethephon); Aminoethoxyvinylglycine; Embryogenic stem cells; Phalaenopsis; Silver nitrate

Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by ... more

Y.W. Huang; Y.J. Tsai; T.C. Cheng; J.J. Chen; F.C. Chen
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The influence of warm day and cool night conditions on induction of spikes in Phalaenopsis orchids has been studied with respect to photosynthetic efficiency, metabolic cycles and physiology. However, molecular events involved in spike emergence induced by warm day and cool night conditions are not clearly understood. We examined gene expression induced by warm day and cool night ... more

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Avian influenza virus; Chicken embryo fibroblast; H9N2; miRNA

There is limited information about microRNAs (miR­NAs) in H9N2 subtype influenza virus-infected chicken cells or tissues. In this study, 10,487,469 and 13,119,795 reads were obtained from in­fected and non-infected chicken embryo fibroblasts, respectively. Seven hundred and thirty-six and 1004 miRNAs, including mature miRNAs and precursors, were obtained from the infected and non-infected ... more

X. Peng; Q.S. Gao; L. Zhou; Z.H. Chen; S. Lu; H.J. Huang; C.Y. Zhan; M. Xiang
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Expression pattern; Flower bud; Male sterility; miRNA

MicroRNAs (miRNAs) are a class of non-coding endogenous negative regulators that regulate gene expression at both the transcriptional and post-transcriptional levels. However, little is known about the expression characteristics of miRNAs during pollen development in Brassica oleracea. In this study, five known and three novel miRNAs were identified and their expression patterns were ... more

J.H. Song; J. Yang; F. Pan; B. Jin
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The giant panda (Ailuropoda melanoleuca) is one of the world’s most endangered mammals, and it has evolved several unusual biological and behavioral traits. During puberty, pregnancy, lactation, and involution, the mammary gland undergoes profound morphological and functional changes. A large number of microRNAs (miRNAs) have been identified to be involved in mammary gland ... more

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Breast cancer; ILIA; Insertion/deletion; miRNA; Polymorphism

The insertion/deletion polymorphism (rs3783553 TTCA/-) in the 3' untranslated region of interleukin-1A (IL1A) has been studied intensively and has been shown to affect tumor risk. We studied the frequency of the IL1A gene polymorphism rs3783553 and evaluated its relationship with breast cancer (BC). A hospital-based case-control study comprising 228 patients with histologically ... more

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Dinoprostone; mRNA; Prostaglandin receptors; Term pregnancy; Uterus

Prostaglandin E2 (dinoprostone) is largely used for labor induction. However, one-third of patients do not respond to treatment. One cause of this poor response may be associated with changes in regulation of prostaglandin E receptors (EP1-4). In this study, we investigated EP mRNA expression in the uterine cervix and lower uterine segment myometrium for term births. Biopsies were ... more

C.K. Konopka; W.G. Glanzner; M.L. Rigo; M.T. Rovani; F.V. Comim; P.B.D. Gonçalves; E.N. Morais; A.Q. Antoniazzi; C.F. Mello; I.B.M. Cruz
11/13/2015
Bronchial asthma; Children; Eosinophils; ECP; mRNA

Studies have shown that eosinophils are closely related to pathogenesis of bronchial asthma. Eosinophils release eosinophil cationic protein (ECP), which plays an important role in infection and allergic reactions. Serum ECP mRNA expression in children with bronchial asthma has not been adequately investigated. We analyzed serum ECP mRNA expression in 63 children with bronchial asthma and ... more

H.Y. Yu; X.Y. Li; Z.F. Cai; L. Li; X.Z. Shi; H.X. Song; X.J. Liu
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Arbovirus; Dengue; DGCR8; mRNA; RNAi

Dengue virus (DENV) and its four serotypes (DENV1-4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and negatively regulate host or viral gene expression. During ... more

S.M.M. Casseb; D.B. Simith; K.F.L. Melo; M.H. Mendonça; A.C.M. Santos; V.L. Carvalho; A.C.R. Cruz; P.F.C. Vasconcelos; S.M.M. Casseb; D.B. Simith; K.F.L. Melo; M.H. Mendonça; A.C.M. Santos; V.L. Carvalho; A.C.R. Cruz; P.F.C. Vasconcelos

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