Research Article

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3/22/2017
Animals; Aphids; Biological evolution; Genetic variation; Hemiptera; Parthenogenesis; Photoperiod; Reproduction, Asexual; RNA; Transcriptome

Chinese galls form on sumac plants (Rhus chinensis) and are used for medicinal and chemical purposes, due to their richness in tannins. The galls are formed by aphids, the most prominent of which is Schlechtendalia chinensis, which forms horn-shaped galls on the winged rachis of R. chinensis. S. chinensis has a complex life cycle, including both R. chinensis and certain mosses as hosts, as ... more

P. Liu; Z.X. Yang; X.M. Chen; P. Yang
4/05/2017
Arthritis, Rheumatoid; Gene expression profiling; Humans; Models, Genetic; Monte Carlo method; Signal transduction; Transcriptome

We attempted to identify significant pathway cross-talk in rheumatoid arthritis (RA) by the Monte Carlo cross-validation (MCCV) method. We therefore obtained and preprocessed the gene expression profile of RA. MCCV involves identifying differentially expressed genes (DEGs), identifying differential pathways (DPs), calculating the discriminating score (DS) of the pathway cross-talk, and random ... more

W. Song; Y.M. Zhang; T. Ma; J. Wang; K.Z. Wang
05/10/2017
Alleles; Animals; Expressed sequence tags; Genetic markers; Heterozygote; Microsatellite Repeats; Perciformes; Polymorphism, Genetic; Transcriptome

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two ... more

P.P. Wang; M. Ren; S.Q. Chen; S.W. Yin; C. Zhao; H.Y. Zhang; X.R. Li; Q.Q. Cao; G.Q. Zhou
05/25/2017
Droughts; Gene Expression Regulation, Plant; Osmotic pressure; Plant Leaves; Saccharum; Transcriptome

Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and ... more

A.A. Belesini; F.M.S. Carvalho; B.R. Telles; G.M. de Castro; P.F. Giachetto; J.S. Vantini; S.D. Carlin; J.O. Cazetta; D.G. Pinheiro; M.I.T. Ferro
09/21/2017
Animals; Chromosomes; Female; Genotype; Hybridization, Genetic; Karyotype; Liver; male; Microsatellite Repeats; Polymorphism, Genetic; Quantitative Trait, Heritable; Transcriptome; Turtles

Chinese soft-shelled turtle Pelodiscus sinensis has been an important aquaculture species in Southeast Asian countries. To breed a new variety of soft-shelled turtle with excellent properties and to evaluate the effect of hybridization of two turtle strains with a highly different trait phenotype, inheritance, microsatellite loci, and transcriptome analysis were studied in the hybrid turtles ... more

H.Q. Zhang; X.J. Xu; Z.Y. He; J.Z. Shao; X.H. Zhang; Q.H. Meng; F.Y. Huang
04/12/2018
Complete metamorphosis; Differentially expressed genes (DEGs); Papilio polytes; Transcriptome

The butterflies are one of the insect groups which undergo typical complete metamorphosis in their life history. However, up to now, little is known about the genomic mechanism that regulates the butterfly metamorphosis. In this study, using a swallowtail butterfly P. polytes as a model organism, a high-throughput sequencing platform was employed to perform the ... more

S. Yao; Yliang Wang; Rsong Tao; Cyong Su; Jsheng Hao
07/31/2020
Magnaporthe oryzae; Oryza sativa; Transcriptome; Weighted gene co-expression network; WGCNA

Rice blast disease is a major threat to rice production worldwide; the causative pathogenic fungus Magnaporthe oryzae induces rice (Oryza sativa) plants to undergo molecular changes that help them to circumvent this fungal attack. Transcriptome studies have demonstrated that many genes are involved in the defense response of rice to M. oryzae ... more

R. Bevitori; S. Sircar; R.N. de Mello; R.C. Togawa; M.V.C.B. Côrtes; T.S. Oliveira; M.F. Grossi-de-Sá; N. Parekh
12/28/2021
Gene expression; Ipomoea batatas; RT-qPCR; Transcriptome

Considering the great economic and social importance of sweet potato and the few transcriptome studies carried out on this species so far, which can potentially lead to significant improvements in its production system at both productivity and quality levels, this study aimed to determine the most suitable methodology for extracting high-quality RNA from sweet ... more

R.C. Gonçalves; M.M. Daude; M.R. de Souza; M.B.F. Moraes; R.O. Moreira; M.A. da Silveira; S.A. Ságio; H.G. Barreto
10/05/2015
Chloroplast microsatellite; Gossypium; Polymorphism; Transferability

Cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. Sixty chloroplast microsatellites were identified and characterized from the complete sequence of the Gossypium hirsutum chloroplast genome using a bioinformatic approach. Twenty chloroplast microsatellite loci were polymorphic in the 66 ... more

X.Y. Cai; F. Liu; Z.L. Zhou; X.X. Wang; C.Y. Wang; Y.H. Wang; K.B. Wang
03/04/2016
Gabiroba; Genetic diversity; SSR; Transferability

We assessed the transferability of 120 EST-derived Eucalyptus microsatellite primers to Campomanesia adamantium and C. pubescens. Both species are berry trees native to the Brazilian Cerrado, and population genetic information is poor. Twelve markers were used to analyze the genetic variability of four sampled populations. Regarding DNA extraction, we sampled ... more

E.A.G.C. Miranda; C.R.D. Boaventura-Novaes; R.S. Braga; E.F. Reis; J.F.N. Pinto; M.P.C. Telles; E.A.G.C. Miranda; C.R.D. Boaventura-Novaes; R.S. Braga; E.F. Reis; J.F.N. Pinto; M.P.C. Telles

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