Research Article

Related GMR Articles

04/15/2003
Goat; Human G-CSF; Pronuclear embryos; Transgenesis

This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was synchronized by a standard progestagen treatment and ... more

V.J.F. Freitas; I.A. Serova; L.E. Andreeva; E.S.Lopes Júnior; D.I.A. Teixeira; M.F. Cordeiro; D. Rondina; N.R.O. Paula; I.J. Arruda; J.B.Lima Verde; G. Dvoriantchikov; O. Serov
03/17/2006
Goat; Lectin; Seminal plasma; Spermadhesin

Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we ... more

D.Ítalo Alv Teixeira; L.Magalhães Melo; C.Alberto de Gadelha; R.Maranguape da Cunha; C. Bloch; G. Rádis-Baptista; B.Sousa Cavada; V.José de F. Freitas
09/01/2009
Caseous lymphadenitis; Corynebacterium pseudotuberculosis; Goat; Sheep; Vaccine
F.A. Dorella
02/03/2015
Cattle; Cross-reactivity; Cytokines; Goat; Human; Sheep

Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated ... more

E.M.S. Dorneles; M.S.S. Araújo; A. Teixeira-Carvalho; O.A. Martins-Filho; A.P. Lage
08/07/2006
Candidate genes; Cattle; DGAT1; PCR-RFLP; Zebu

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection ... more

G.A. Lacorte; M.A. Machado; M.L. Martinez; A.L. Campos; R.P. Maciel; R.S. Verneque; R.L. Teodoro; M.G.C.D. Peixoto; M.R.S. Carvalho; C.G. Fonseca
08/18/2009
Candidate gene; Genotyping; PCR-RFLP; POU1F

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme RsaI. The data were ... more

A.C.P. Silveira; T.F. Braga; J.F. Almeida; R.C. Antunes; P.F.A. Freitas; A.S.M. César; A.S.M. César; E.C. Guimarães
10/28/2008
12S/16S gene; gene variation; Microchiroptera; PCR-RFLP; RAG2 gene

A PCR-RFLP analysis of the restriction pattern in nuclear (RAG2) and mitochondrial (12S/16S) gene sequences of bat species from the Molossidae, Phyllostomidae, Vespertilionidae, and Emballonuridae families produced a large number of fragments: 107 for RAG2 and 155 for 12S/16S combined in 139 and 402 haplotypes, respectively. The values detected for gene variation were low for both sequences (0 ... more

S.R.C. Marchesin; M.R. Beguelini; K.C. Faria; P.R.L. Moreira; E. Morielle-Versute
10/31/2011
Melanocytes; Oxidative stress catalase gene (CAT); PCR-RFLP; Vitiligo

Accumulation of hydrogen peroxide (H2O2) and low catalase (CAT) activity have been demonstrated in the epidermis of vitiligo patients. We investigated a possible association between the CAT exon 9 (Asp-389) gene and vitiligo susceptibility in the Turkish population. Thirty-four patients with vitiligo and 49 gender, age and ethnic matched controls were ... more

H. Bulut; M. Pehlivan; S. Alper; A.G. Tomatir; H. Onay; S.E. Yüksel; F. Özkinay; S. Pehlivan
11/04/2011
AGPAT6 gene; Dairy goat; Milk traits; PCR-RFLP; Polymorphism

As one of the eight members in the 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs) family, AGPAT6 is a crucial enzyme for the biosynthesis of glycerolipids and triacylglycerol in eukaryotes, as well as catalyzing the conversion from lysophosphatidic acid to phosphatidic acid. AGPAT6 can be considered as a candidate gene for regulating milk composition. DNA ... more

C. He; C. Wang; Z.H. Chang; B.L. Guo; R. Li; X.P. Yue; X.Y. Lan; H. Chen; C.Z. Lei
07/25/2011
PCR-RFLP; Porcine stress syndrome gene; PSE; Swine

We evaluated the effect of the halothane (HAL) gene on the quality of pork in domestic pigs. Half-carcasses from two different commercial pig (Sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (HALNN) and 69 of which were heterozygous (HALNn) for the halothane gene. The measures included backfat thickness, lean meat percentage, ... more

A.C.P. Silveira; P.F.A. Freitas; A.S.M. César; A.S.M. César; R.C. Antunes; E.C. Guimarães; D.F.A. Batista; L.C. Torido

Pages