Research Article

Related GMR Articles

Body measurement traits; Goat; LF gene; Milk composition; PCR-SSCP; SNP

Milk composition and body measurement traits, influenced by genes and environmental factors, play important roles in value assessments of efficiency and productivity in dairy goats. Lactoferrin (LF), involved in the efficient expression of protein in milk, is also an anabolic factor in skeletal tissue and a potent osteoblast survival factor. Therefore, it is an important candidate gene ... more

B.L. Guo; Y. Jiao; C. He; L.X. Wei; Z.H. Chang; X.P. Yue; X.Y. Lan; H. Chen; C.Z. Lei
Association analysis; Genetic variation; Goat; Production traits; Sine oculis homeobox homolog 6 (Six6) gene

We looked for novel genetic variations within the Six6 gene by PCR-SSCP, DNA sequencing and forced RFLP-PCR and estimated their associations with production traits in 2132 goats of eight indigenous Chinese breeds. A novel single nucleotide polymorphism (NM_001104993.1: g.232T>C) within the goat Six6 gene was identified. The frequencies of allele “C” varied from 0.8621 ... more

C.Y. Pan; X.Y. Lan; H.Y. Zhao; S.R. Hu; Y.T. Huai; C.Z. Lei; H. Chen
Goat; MicroRNA characteristics; Real-time quantitative PCR; Solexa deep-sequencing technology

MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this study, small RNA cDNA libraries of ... more

Y.H. Ling; J.P. Ding; X.D. Zhang; L.J. Wang; Y.H. Zhang; Y.S. Li; Z.J. Zhang; X.R. Zhang
Goat; Growth traits; Myostatin gene (MSTN); PCR-SSCP; Polymorphism

Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for ... more

Z.J. Zhang; Y.H. Ling; L.J. Wang; Y.F. Hang; X.F. Guo; Y.H. Zhang; J.P. Ding; X.R. Zhang
DNA extraction; Goat; PCR; semen

Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNeasy Blood & Tissue Kit, phenol-chloroform, and Chelex-100 ... more

E.C.B. Silva; M.A. Pelinca; A.C. Acosta; D.M.F. Silva; M.A.Gomes Filho; M.M.P. Guerra
Genetic structure; Goat; Microsatellite marker; Phylogeny

The present study aimed to analyze genetic relatedness and differentiation of common native goat populations in some countries of the Middle East. The populations were Ardi, Black Bedouin, and Damascus goats in the Kingdom of Saudi Arabia, Jordan, and Syria, respectively. Domesticated goats of the Middle East are mostly related to common ancestors, but there is limited molecular genetic ... more

R.M. Al-Atiyat; R.S. Aljumaah
Cattle; Meat quality traits; MYPN gene; PCR-RFLP; SNP

Myopalladin (MYPN) is a multifunctional protein that maintains sarcomeric integrity and regulates Z-line structure. It is an important candidate gene for meat quality selection through marker-assisted selection. Using PCR-RFLP technology, we discovered a single-nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene. Allele frequencies of this SNP were investigated and evaluated ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang; B.L. Guo
Clone; Duck; PCR-RFLP; PLIN gene; SNP

The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, ... more

H.L. Zhang; H.J. Fan; X.L. Liu; Y. Wu; S.S. Hou
Cattle; CDIPT gene; PCR-RFLP; SNP; Ultrasound measurement traits

The CDIPT is crucial to the fatty acid metabolic pathway, intracellular signal transduction and energy metabolism in eukaryotic cells. We detected three SNPs at 3'-untranslated regions (UTR), named 3'-UTR_108 A > G, 3'-UTR_448 G > A and 3'-UTR_477 C > G, of the CDIPT gene in 618 Qinchuan cattle using PCR-RFLP and DNA sequencing methods. At each of the three SNPs, we ... more

C.Z. Fu; H. Wang; C.G. Mei; J.L. Wang; B.J. Jiang; X.H. Ma; H.B. Wang; G. Cheng; L.S. Zan

We investigated the polymorphisms of PRLR and FOLR1 genes in Xinong Saanen, Guanzhong, and Boer goat breeds by DNA sequencing and PCR–RFLP. Two novel SNPs were identified: KC109741: g.62130C>T in the 3ꞌ-UTR of goat gene PRLR, and KC136296: g.7884A>C in exon 3 of goat gene FOLR1. In the three goat breeds, the polymorphism information content was 0. ... more

J.X. Hou; F. Fang; X.P. An; Y. Yan; T. Ma; P. Han; F.X. Meng; Y.X. Song; J.G. Wang; B.Y. Cao