Research Article

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16S rRNA; Endophytic bacterium; Microbial diversity; Sugarcane

Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium was used to cultivate the endophytes; 32 isolates were selected ... more

G.S. Magnani; C.M. Didonet; L.M. Cruz; C.F. Picheth; F.O. Pedrosa; E.M. Souza
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16S rRNA; COI; Mitochondrial DNA gene sequences; Molecular phylogeny; Octopodidae species

Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c ... more

Z.M. Lü; W.T. Cui; L.Q. Liu; H.M. Li; C.W. Wu
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16S rRNA; Bacterial consortium; DNA extraction; Electrical energy; Inexpensive DNA protocol; Microbial fuel cell

Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs. ... more

B. Canto-Canché; M. Tzec-Simá; J.I. Vázquez-Loría; H. Espadas-Álvarez; B.H. Chí-Manzanero; R. Rojas-Herrera; R. Valdez-Ojeda; L. Alzate-Gaviria
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16S rRNA; Diversity; ERIC-PCR; High-throughput screening; Proteobacteria; RAPD

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia ... more

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16S rRNA; Anaerobic biodigestion; Microbial community; Support medium

The performance of anaerobic filter bioreactors (AFs) is influenced by the composition of the substrate, support medium, and the microbial species present in the sludge. In this study, the efficiency of a slaughterhouse effluent treatment using three AFs containing different support media was tested, and the microbial diversity was investigated by amplified ribosomal DNA restriction ... more

M.I. Stets; R.M. Etto; C.W. Galvão; R.A. Ayub; L.M. Cruz; M.B.R. Steffens; A.C. Barana
05/09/2014
16S rRNA; COI; Crustacean; Genetic polymorphism; Genetic variability; Macrobrachium

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and ... more

A.L. Guerra; A.V.B. Lima; R.V.Lucato Júnior; M.C. Chiachio; F.G. Taddei; L. Castiglioni
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16S rRNA; Biotechnology; DNA barcode; Meliaceae; Microbiome

Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide dispersion in South ... more

S.A. Rhoden; A. Garcia; M.C.Santos e Silva; J.L. Azevedo; J.A. Pamphile
09/27/2013
Endophytic fungi; Esterases; Isoenzymes; Molecular markers; rDNA; Sugarcane

This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with α-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.8S-ITS2 sequencing data were used as ... more

A.C. Leme; M.R.R. Bevilaqua; S.A. Rhoden; C.A. Mangolin; M.F.P.S. Machado; J.A. Pamphile
05/08/2012
Genetic identity; Polymerase chain reaction; Simple sequence repeat; Sugarcane

Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers were used for DNA fingerprinting and genetic diversity ... more

U. Hameed; Y.B. Pan; K. Muhammad; S. Afghan; J. Iqbal
11/24/2009
Endophytic bacteria; Erwinia; Pantoea; Plant-bacteria interaction

Endophytic bacteria were isolated from stems of Eucalyptus spp (Eucalyptus citriodora, E. grandis, E. urophylla, E. camaldulensis, E. torelliana, E. pellita, and a hybrid of E. grandis and E. urophylla) cultivated at two sites; they were characterized by RAPD and amplified rDNA restriction analysis (ARDRA). ... more

R.E.L. Procópio; W.L. Araújo; W. Maccheroni; J.L. Azevedo

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