Research Article

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08/05/2011
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The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mammalian nucleotide sequence. RT-PCR and PCR ... more

W.R. Hou; Y.L. Hou; G.F. Wu; Y. Song; X.L. Su; B. Sun; J. Li
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Ailuropoda melanoleuca; Cloning; FABP4 and FABP5; Genomic sequence; Giant panda; Overexpression

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) ... more

B. Song; Y.L. Hou; X. Ding; T. Wang; F. Wang; J.C. Zhong; T. Xu; J. Zhong; W.R. Hou; S.R. Shuai
01/25/2011
Ailuropoda melanoleuca; cDNA cloning; Giant panda; Overexpression; RPS13 gene; Sequence analysis

The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared ... more

Y. Song; Y.L. Hou; W.R. Hou; G.F. Wu; T. Zhang
10/13/2010
Ailuropoda melanoleuca; cDNA cloning; Genomic cloning; Giant panda; Overexpression; RPS14

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was ... more

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09/03/2012
Ailuropoda melanoleuca; ATP5G1; cDNA cloning; Giant panda; Overexpression

The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp encoding ... more

W.R. Hou; Y.L. Hou; X. Ding; T. Wang
11/02/2008
cDNA; Expressed sequence tags; heavy metal; woody plant

Many cell functions are redundantly executed in cells, and the experimental approaches that analyze the group of proteins, whose expression is modified in a specific functional condition, enable the identification of the group of proteins that are expressed under stress conditions. The objective of the present study was the evaluation of the genetic expression induced by cadmium (Cd) in ... more

V.L. Souza; A.A.F. Almeida; B.T.Hora Júnior; B.T.Hora Júnior; A.S. Gesteira; J.C.M. Cascardo
05/18/2015
Broad-complex; cDNA; Macrobrachium nipponense; Metamorphosis; Real-time quantitative polymerase chain reaction

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends ... more

S.F. Jiang; Y.P. Zhang; S.M. Sun; Y.S. Gong; Y.W. Xiong; H. Qiao; W.Y. Zhang; S.B. Jin; H.T. Fu
03/31/2015
Anthocyanin; Berry; cDNA; Flavonoid; Gene expression

Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase gene, was cloned from ... more

X.G. Li; J. Wang; Z.Y. Yu
10/31/2011
Cloning; Gene expression; Granulocyte colony-stimulating factor; Recombinant G-CSF; Synthetic cDNA

Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches for the synthesis of this pharmacologically active agent. We ... more

S. Alrokayan
12/14/2010
Cloning; Litopenaeus vannamei; Ryanodine receptor; Single-nucleotide polymorphism

Ryanodine receptor/calcium release channel is a large protein that plays an essential role in muscle contraction; mutations in the ryanodine receptor gene affect sensitivity to stress. As a first step towards investigating the relationship between the ryanodine receptor and shrimp cramped muscle syndrome, we cloned, partially sequenced, and examined single-nucleotide polymorphisms (SNPs) ... more

X.H. Chen; D.G. Zeng; N. Ma

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