Research Article

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08/30/2011
Canopy form; Cypress; Fastigate; Pseudo-test cross; RAPD; SCAR

Gene mapping for a Cupressus species is presented for the first time. Two linkage maps for the Mediterranean cypress (Cupressus sempervirens) varieties, C. sempervirens var. horizontalis and C. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological ... more

C. Manescu; Ν. Hamamouch; C. Maios; A. Harfouche; A.G. Doulis; F.A. Aravanopoulos
08/29/2012
Liriope; Ophiopogon; RAPD; SCAR; Species discrimination

The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to detect ... more

G. Li; Y.J. Park
02/24/2012
Cultivar discrimination; DNA marker; RAPD; Rosa; SCAR

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. ... more

S. Riaz; B. Sadia; F.S. Awan; I.A. Khan; H.A. Sadaqat; I.A. Khan
05/30/2014
Cloning; RAPD; Rose; SCAR; Sequencing

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is ... more

K.M.I. Bashir; F.S. Awan; I.A. Khan; A.I. Khan; M. Usman
06/14/2011
Chlorophyll accumulation; RAPD; Relative electrolyte leakage; SDS-PAGE

Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of heat-tolerant genotypes so that only the best are chosen ... more

R.M. Rana; S.H. Khan; Z. Ali; A.I. Khan; I.A. Khan
05/17/2011
Genetic diversity; Mycelial compatibility groups; RAPD; Sclerotinia sclerotiorum; White mold

The genetic variability of 40 Sclerotinia sclerotiorum isolates from various fields widely distributed throughout Brazil and different host crops was analyzed using RAPD markers and mycelial compatibility groupings (MCGs). The isolates were characterized using 16 random primers of the OPERON series, which produced 121 DNA fragments. UPGMA cluster analysis using Jaccard’s genetic ... more

C.G.Litholdo Júnior; E.V. Gomes; L. Júnior; L.C.B. Nasser; S. Petrofeza
02/23/2010
Genotype; RAPD; Resistance; Varroa destructor

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility in different geographical regions. Information about ... more

J.C.V. Guerra; M.R.C. Issa; F.E. Carneiro; R. Strapazzon; G. Moretto
10/21/2008
Bos indicus; Bos taurus; DNA marker; marker-assisted selection; Microsatellite; Nelore

The aim of the present article was to study the population structure and genetic diversity of Nelore cattle and genetic relationships between Nelore and different taurine and zebu breeds raised in Brazil. DNA polymorphism analysis was carried out with 1976 animals of 16 zebu, taurine and synthetic breeds raised in Brazil. A higher genetic differentiation was observed in taurine than in zebu ... more

M.A.C. Dani; M.B. Heinneman; S.U. Dani
10/30/2013
Bayesian inference; marker-assisted selection; Molecular breeding value

The availability of molecular marker information has turned out to be an opportunity to improve animal breeding programs, by the inclusion of those effects in the estimation of breeding values. Along this perspective, the aims of present research were to compare genetic evaluation models that assume or not marker effects in the estimation of breeding values, as well as to estimate the ... more

F.M. Rezende
08/12/2013
Bos indicus; marker-assisted selection; Molecular breeding value; Protein turnover; Residual feed intake

We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, ... more

R.C. Gomes; S.L. Silva; M.E. Carvalho; F.M. Rezende; L.F.B. Pinto; M.H.A. Santana; T.R. Stella; F.V. Meirelles; R. Júnior; P.R. Leme; J.B.S. Ferraz

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