Research Article

Related GMR Articles

05/23/2014
Asterias rubens; Gene expression; High-throughput sequencing; Real-time qPCR; Reference gene; UBA52

The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas. This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression at the transcriptional level is estimated by real- ... more

A.F. Sadritdinova; A.A. Dmitriev; A.V. Snezhkina; M.S. Belenikin; G.S. Krasnov; O.G. Manylov; A.A. Kudryavtsev; N.V. Melnikova; A.S. Speranskaya; M.V. Darii; V.A. Lakunina; L.A. Uroshlev; A.O. Smurov; O.A. Stepanov; A.V. Kudryavtseva
04/14/2014
Carcass trait; Gene expression; Hu sheep; IGF-I; MyoG

In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.01 < P < 0.05), one-month old (0.01 < P < 0.05), ... more

W. Sun; R. Su; D. Li; H.H. Musa; Y. Kong; J.T. Ding; Y.H. Ma; L. Chen; Y.F. Zhang; W.Z. Wu
01/22/2014
5-aminolevunlinic acid; Aquaporin; Cucumber; Gene expression; NaCl stress

Aquaporins play a direct role in plant water relation under salt stress, but the effects of 5-aminolevulinic acid (ALA) on aquaporin gene expression in salt-treated plants remain unknown. This study investigated the potential effects of exogenous ALA (50 mg/dm3) on aquaporin expression levels under salt stress (75 mM NaCl) in the salt-sensitive (Jinchun No.4) and the relatively salt- ... more

F. Yan; D. Qu; Y.Y. Zhao; X.H. Hu; Z.Y. Zhao; Y. Zhang; Z.R. Zou
01/22/2014
Gene expression; Gibberellin; Oilseed rape; Reserve accumulation; Seed filling

Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA ... more

X.Q. Huang; R.Q. He; X.Y. Liao; B. Zhou; W.S. Peng; J.Z. Lin; D.Y. Tang; Y.H. Zhu; X.Y. Zhao; X.M. Liu
02/20/2014
Adipose; Evodiamine; Gene expression; Long-term effect; Metabolism

Evodiamine, the major alkaloid component isolated from the fruit of dried, unripened Evodia rutaecarpa Bentham, affects the plasma levels of cholecystokinin and various biological events such as gastric emptying and gastrointestinal transit; these effects of evodiamine were previously investigated in male rats. In this study, we aimed to investigate the effects of evodiamine on ... more

D.F. Jiang; W.T. Li; H.L. Yang; Z.Z. Zhang; D. Chen; C. Sun
02/13/2014
Endogenous genes; Flooding; Gene expression; Housekeeping genes; Internal control genes

Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the ... more

T.J. Nakayama; F.A. Rodrigues; N. Neumaier; F.C. Marcelino-Guimarães; J.R.B. Farias; M.C.N. de Oliveira; A. Borém; A.C.B. de Oliveira; B.M. Emygdio; A.L. Nepomuceno
02/19/2014
Crucian carp; Distant hybridization; Gene expression; jnk1 gene; Molecular cloning; Polyploidy

c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility of both female and male animals. In this study, 2 jnk1 ... more

Y.M. Xiao; M.G. Jiang; Z.W. Luo; Y.H. Zhou; S. Wen; M. Wang; C. Zhang; S.J. Liu
06/26/2015
Adipose tissue; Conjugated linoleic acid; Gene expression; MicroRNA; Pig

In this study, we evaluated the effect and possible mech­anism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were col­lected, and real-time polymerase chain reaction (PCR) was ... more

R.L. Qi; Y. Chen; J.X. Huang; F.Y. Yang
06/18/2015
Gene expression; Partial least squares; Preeclampsia

Preeclampsia is major cause of maternal and fetal morbidity and mortality. Currently, the etiology of preeclampsia is unclear. In this study, we investigated differences in gene expression between preeclampsia patients and controls using partial least squares-based analysis, which is more suitable than routine analysis. Expression profile data were downloaded from the Gene Expression ... more

F. Jiang; Y. Yang; J. Li; W. Li; Y. Luo; Y. Li; H. Zhao; X. Wang; G. Yin; G. Wu
05/18/2015
Ankylosing spondylitis; Gene expression; Meta-analysis; Pathway analysis

The purpose of this study was to identify differentially expressed (DE) genes and biological processes associated with changes in gene expression in ankylosing spondylitis (AS). We performed a meta-analysis using the integrative meta-analysis of expression data program on publicly available microarray AS Gene Expression Omnibus (GEO) datasets. We performed Gene Ontology (GO) enrichment ... more

Y.H. Lee; G.G. Song

Pages