Research Article

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DNA methylation; Gossypium; introgression lines; Polymorphism

Introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. The DNA methylation level among 10 introgression lines of cotton (Gossypium hirsutum) and three exotic parental species (G. arboreum, G. thurberi and G. barbadense) were assessed by methylation-sensitive amplified ... more

S.P. He; J.L. Sun; X.M. Du
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Chromatin; DNA methylation; FISH; Micrococcal nuclease; Myostatin

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in several tissues (liver, eye, kidney, brain ... more

E.M. Abbas; A. Takayanagi; N. Shimizu; M. Kato
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DNA methylation; Epigenetic inheritance; Molecular marker; Mutation analysis; Plant breeding

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amplification of polymorphic ... more

M.G.F. Rodrigues; A.B.G. Martins; B.W. Bertoni; A. Figueira; S. Giuliatti
04/17/2013
DNA methylation; Osmotic stress; Proline; Rice

Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is associated with DNA methylation ... more

C.Y. Zhang; N.N. Wang; Y.H. Zhang; Q.Z. Feng; C.W. Yang; B. Liu
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DNA methylation; Heat inducible; HSP70

DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation ... more

J.K. Gan; D.X. Zhang; D.L. He; X.Q. Zhang; Z.Y. Chen; Q.B. Luo
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5-Aza-2´-deoxycytidine; APAF-1; Cervical cancer; DNA methylation; RASSF1A

The methylation of tumor suppressor genes has been shown to be involved in many human cancers. 5-Aza-2’-deoxycytidine (5-Aza-CdR) can reactivate the expression of methylated tumor suppressor genes. In our study, 2 human cervical cancer cell lines, HeLa and SiHa, were treated with different concentrations (20, 10, 5, and 2.5 μM) of 5-Aza-CdR for 24, 48, and 72 h. After incubation, cells ... more

T.T. Yao; S.M. Mo; L.Y. Liu; H.W. Lu; M.L. Huang; Z.Q. Lin
09/26/2012
DNA methylation; Fat; MSAP; Muscle; Pig

DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire cross-bred pigs. The degrees of ... more

J.D. Ma; M.Z. Li; S.L. Zhou; C.W. Zhou; X.W. Li
08/29/2012
DNA methylation; Gastric cancer; LOH; MSI; PAI-1 gene

We explored a possible correlation of genetic instability and CpG methylation in the 5'-flanking region of the PAI-1 gene with clinicopathologic features of gastric cancer in Chinese patients and looked for molecular markers for diagnosing gastric tumor development. Microsatellite instability and loss of heterozygosity of the PAI-1 gene locus D7S515, D7S471 and pai-1 ... more

J. Liu; X. Li; N. Yu; Y.Q. Yang; X. Li; Z.Y. Ye; J.C. Li
02/16/2012
DNA methylation; Histone acetylation; PEV; Transgene expression; Transgenic pig

Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or epigenetic modification, plays a more important role in the ... more

Z. Yin; Q.R. Kong; Z.P. Zhao; M.L. Wu; Y.S. Mu; K. Hu; Z.H. Liu
10/27/2014
DNA methylation; Mouse embryo; Paternal genome; Zygote

The developmental dynamics of DNA methylation events have been well studied. Active demethylation of the paternal genome occurs in the zygote, passive demethylation occurs during cleavage stages, and de novo methylation occurs by the blastocyst stage. It is believed that the paternal genome has lower levels of methylation during early development than the maternal genome. ... more

X.S. Ma; X.G. Wang; L. Qin; C.L. Song; F. Lin; J.M. Song; C.C. Zhu; H.L. Liu

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