Research Article

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07/14/2009
Genetic distance; Genome; Microsatellite; Molecular markers; Population genetics; Triticum aestivum

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity ... more

S. Ijaz; I.A. Khan
02/17/2009
Bombyx mori multiple nucleopolyhedrovirus; Molecular markers; Polymorphism; Random amplified polymorphic DNA; Silkworm

The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance to baculovirus infection to analyze the ... more

L.F.C. Ribeiro; D.B. Zanatta; J.P. Bravo; R.M.C. Brancalhão; M.A. Fernandez
09/12/2011
Genetic marker; Larus saundersi; Microsatellite; Polymorphism; Saunders’s gull

Nine polymorphic microsatellite loci were isolated, using tetranucleotide repeat oligonucleotide probes from an enriched DNA library of the globally “vulnerable” Saunders’s gull (Larus saundersi), collected from the Yancheng coastal wetland, one of the three remaining breeding sites in China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight eggshell membrane ... more

H.X. Jiang; S.L. Ren; Y.Q. Hou; M. Yang; X.B. Wu
08/16/2011
Invasive species; Microsatellite; Polymorphism; Pseudorasbora parva

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The ... more

C. Zeng; Y. Gul; K. Yang; L. Cui; W.M. Wang; Z.X. Gao
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Duchenne muscular dystrophy; Dystrophin gene; Han population genetics; Microsatellite; Polymorphism

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and 2nd intron were found to have a high degree of polymorphism. ... more

R.F. Sun; Y.S. Zhu; J.L. Feng; Z. Tian; W.J. Kuang; Y. Liu; H.B. Zhang; S.B. Li
02/15/2011
Genetic marker; Microsatellite; Pelophylax hubeiensis; Polymorphism

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0. ... more

J.C. Yan; F.H. Zhu; H.L. Wu
11/23/2010
DNA fingerprinting; Genetic characterization; Heat tolerance; Molecular markers; Polymorphism; Solanum lycopersicon

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable ... more

N. Comlekcioglu; O. Simsek; M. Boncuk; Y. Aka-Kacar
12/10/2013
Microsatellite; Oplegnathus fasciatus; Polymorphism

The rock bream fish Oplegnathus fasciatus is one of the most popular aquaculture species in China. In the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of O. fasciatus from the Zhoushan coast of China. The number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individuals. Observed and ... more

X.X. Sun; D.D. Xu; B. Lou; S.L. Li; W. Zhan; Y.R. Zhang; J. Xin
12/04/2013
Microsatellite; Nibea albiflora; Polymorphism

Eleven novel microsatellite loci were isolated from a (CA)10-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected ... more

D.D. Xu; B. Lou; S.L. Li; Y.R. Zhang; J. Xin; W. Zhan
08/06/2012
EST-SSRs; Molecular markers; Musella; Polymorphism; Transferability

We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism. A set of 34 ... more

W.J. Li; H. Ma; Z.H. Li; Y.M. Wan; X.X. Liu; C.L. Zhou

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