Research Article

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Acrididae; Gene mapping; Hsp; In situ hybridization; Schistocerca pallens; Single-copy genes

There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent in situ ... more

T.T. Rieger; S.V. Oliveira-Silva; I.A. Pachêco; B.S. Chagas; J.F. Santos
10/20/2009
Germin; In situ hybridization; RT-PCR; Salt stress; Wheat development

The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of plant development, such as defense, embryonic development and ... more

M. Caliskan
11/29/2011
C-banding; In situ hybridization; Leaf rolling; Triticale; X Triticosecale Wittmack

Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed protein showed that CMH83 was genetically stable. ... more

E.N. Yang; Z.J. Yang; J.F. Zhang; Y.C. Zou; Z.L. Ren
10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
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Bacterial artificial chromosomes; Chromosome variation; In situ hybridization; Pepper

Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in Lycopersicon ... more

V. Andrade-Souza; M.G.C. Costa; C.X. Chen; F.G. Gmitter; M.A. Costa
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Gene expression; In situ hybridization; Planarian; Regeneration; STAG

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those ... more

Z.Q. Yuan; B.S. Zhao; J.Y. Zhang
01/23/2015
Cashmere goats; In situ hybridization; Real-time polymerase chain reaction; RORα; RORα expression

The expression of retinoid-acid-related orphan receptor α (RORα) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that RORα ... more

Y.H. Zhao; Z.H. Liu; L. Wang; H.M. Xiao; C.G. Du; Y.J. Zhang; R. Su; J.Q. Li
12/19/2012
Chitin deacetylase gene; Chitinase-3 gene; Expression; Hyriopsis cumingii; Potential functions

Chitin, the second most important natural polymer in the world, and its N-deacetylated derivative chitosan are found in a wide variety of organisms. These versatile biopolymers are associated with a broad range of biological functions. This article is the first to report the potential functions of 2 chitin metabolic enzyme genes from Hyriopsis cumingii. A chitinase-3 gene ( ... more

G.L. Wang; B. Xu; Z.Y. Bai; J.L. Li
08/28/2014
Food availability; Gene expression; Hyriopsis cumingii; α-amylase

The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (HcAmy) was isolated from H. cumingii by the rapid ... more

G. Ren; J.Y. Tang; Y. Wang
01/29/2014
Big defensin; Hyriopsis cumingii; Immune response; Induced expression

Antimicrobial peptides (AMPs), of which big defensins are examples, are an important component of the natural defenses of most living organisms, and possess remarkable microbicidal activities. In the present study, using expressed-sequence tag sequences from a cDNA library and RACE, the full-length cDNA sequence of the big defensin gene from the triangle-shell pearl mussel, Hyriopsis ... more

G.L. Wang; X.L. Xia; X.L. Li; S.J. Dong; J.L. Li

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