Research Article

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11/17/2011
Bos taurus; Carcass traits; Chemerin gene; PCR-SSCP; SNP

Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was ... more

W.Q. Tian; H.C. Wang; F.B. Song; L.S. Zan; H. Wang; H.B. Wang; Y.P. Xin; J.A. Ujan
09/16/2011
Bovine C/EBPa gene; Genetic polymorphism; Meat quality traits; PCR-SSCP

CCAAT/enhancer-binding protein alpha (C/EBPa) is an essential transcriptional factor regulating the differentiation of adipocytes. We report a novel single nucleotide polymorphism (C271A) of the C/EBPa gene in six indigenous Chinese cattle breeds using PCR-SSCP and DNA sequencing methods. Allele frequencies were investigated and evaluated by the χ2 test in 817 individuals; all ... more

H. Wang; L.S. Zan; H.B. Wang; F.B. Song
09/09/2011
ACOX1 gene; Cattle; PCR-SSCP; SNP; Ultrasound traits

Acyl-coenzyme A oxidase 1 (ACOX1) is the first enzyme in peroxisomal fatty acid β-oxidation; it is rate-limiting and plays a key role in fatty acid metabolism and fat deposition. ACOX1 is an important candidate gene for meat quality selection through marker-assisted selection. Genomic structural analysis showed that bovine ACOX1 shares 86% identity with human ACOX1. Using PCR-SSCP ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang
08/16/2011
IGF-1; PCR-SSCP; SNP

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, ... more

L.L. Hao; H. Yu; Y. Zhang; S.C. Sun; S.C. Liu; Y.Z. Zeng; Y.X. Ai; H.Z. Jiang
04/19/2011
Cattle; Growth traits; PCR-SSCP; SNPs; SST gene

Somatostatins play a crucial role in the regulation of growth and development in vertebrates, especially muscle growth. We assessed the association of somatostatin gene polymorphisms with growth traits by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and DNA sequencing methods in 694 individuals from six Chinese cattle breeds. A novel single nucleotide ... more

L. Gao; L.S. Zan; H.B. Wang; R.J. Hao; X. Zhong
11/09/2010
Body measurement traits; Goat; LF gene; Milk composition; PCR-SSCP; SNP

Milk composition and body measurement traits, influenced by genes and environmental factors, play important roles in value assessments of efficiency and productivity in dairy goats. Lactoferrin (LF), involved in the efficient expression of protein in milk, is also an anabolic factor in skeletal tissue and a potent osteoblast survival factor. Therefore, it is an important candidate gene ... more

B.L. Guo; Y. Jiao; C. He; L.X. Wei; Z.H. Chang; X.P. Yue; X.Y. Lan; H. Chen; C.Z. Lei
02/28/2013
Body measurement traits; DKK4 gene; PCR-SSCP; Qingchuan cattle; SNPs

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of bovine Dickkopf homolog 4 (DKK4) are associated with body measurement traits in Qinchuan cattle. By using PCR-SSCP technology and DNA sequencing, we discovered 5 DKK4 SNPs in Qingchuan cattle, including -65G>A and -77G>T in the 5ꞌ-untranslated region, 1532C>G and 1533T>C ... more

J.B. Gao; Y.K. Li; N. Yang; X.H. Ma; C. Adoligbe; B.J. Jiang; C.Z. Fu; G. Cheng; L.S. Zan
04/02/2013
Goat; Growth traits; Myostatin gene (MSTN); PCR-SSCP; Polymorphism

Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for ... more

Z.J. Zhang; Y.H. Ling; L.J. Wang; Y.F. Hang; X.F. Guo; Y.H. Zhang; J.P. Ding; X.R. Zhang
08/29/2012
ANAPC13; Body measurement traits; Cattle; PCR-SSCP; Polymorphism

Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. There has been some indication that ANAPC13 influences adult height. We used PCR-SSCP and DNA sequencing technology to identify polymorphisms in the ANAPC13 gene. A polymorphism in intron 1 (A > G at base 17) was identified and an ... more

B.J. Jiang; X.L. Zhan; C.Z. Fu; H.B. Wang; G. Cheng; L.S. Zan
06/21/2012
ARMS-PCR; Genotyping; mutations; PCR-SSCP; PRNP alleles; Sheep

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from ... more

C.L. Zhao; R. Wu; L. Liu; F.D. Li; X.L. Zhang; C. Wang; F. Wang; X.L. Diao; H.W. Guan; X. Wang; L. Zhou

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