Research Article

Related GMR Articles

04/25/2013
Green fluorescent protein; Microbubble; Recombinant adeno-associated virus; Ultrasound

Ultrasound-targeted microbubble destruction (UTMD) has been recently developed for destroying bubbles carrying drugs or genes, thereby permitting local release of these target molecules. We in­vestigated whether SonoVue®, a new contrast agent that contains phos­pholipid-stabilized microbubbles filled with sulfur hexafluoride vapor, is effective at delivering a recombinant adeno ... more

S.L. Yang; Y.M. Mu; K.Q. Tang; X.K. Jiang; W.K. Bai; E. Shen; B. Hu
08/05/2011
cDNA; Cloning; Genomic sequence; Giant panda (Ailuropoda melanoleuca); Overexpression; Ribosomal protein gene L9 (rpL9)

The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mammalian nucleotide sequence. RT-PCR and PCR ... more

W.R. Hou; Y.L. Hou; G.F. Wu; Y. Song; X.L. Su; B. Sun; J. Li
01/25/2011
Ailuropoda melanoleuca; cDNA cloning; Giant panda; Overexpression; RPS13 gene; Sequence analysis

The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared ... more

Y. Song; Y.L. Hou; W.R. Hou; G.F. Wu; T. Zhang
10/13/2010
Ailuropoda melanoleuca; cDNA cloning; Genomic cloning; Giant panda; Overexpression; RPS14

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was ... more

G.F. Wu; Y.L. Hou; W.R. Hou; Y. Song; T. Zhang
12/04/2013
miR-212; miRNAs; Overexpression; Target genes

MicroRNAs (miRNAs) are a type of non-protein-coding single-stranded RNA, which are typically 20-25 nt in length. miRNAs play important roles in various biological processes, including development, cell proliferation, differentiation, and apoptosis. We aimed to detect the miRNA response to cocaine stimulations and their target genes. Using the miRNA expression data GSE21901 downloaded from ... more

L.F. Xu; J. Wang; F.B. Lv; Q. Song
12/02/2013
Bone mesenchymal stem cells; OCT4; Overexpression; SOX2; Xiaomeishan porcine

Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify ... more

Y.X. Fan; C.H. Gu; Y.L. Zhang; B.S. Zhong; L.Z. Wang; Z.R. Zhou; Z.Y. Wang; R.X. Jia; F. Wang
10/18/2013
Anticancer activity; cDNA; Cloning; Giant panda; Overexpression; Purification; Ribosomal protein L24

The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain reaction technology ... more

Y.L. Hou; X. Ding; W. Hou; B. Song; T. Wang; F. Wang; J. Li; J. Zhong; T. Xu; B.X. Ma; H.Q. Zhu; J.H. Li; J.C. Zhong
09/03/2012
Ailuropoda melanoleuca; ATP5G1; cDNA cloning; Giant panda; Overexpression

The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp encoding ... more

W.R. Hou; Y.L. Hou; X. Ding; T. Wang
02/20/2014
Ailuropoda melanoleuca; Cloning; FABP4 and FABP5; Genomic sequence; Giant panda; Overexpression

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) ... more

B. Song; Y.L. Hou; X. Ding; T. Wang; F. Wang; J.C. Zhong; T. Xu; J. Zhong; W.R. Hou; S.R. Shuai
03/31/2015
Cattle A-FABP; Fat deposition; Overexpression; Transgenic mice

Adipocyte fatty acid-binding protein (A-FABP), the most abundant FABP in adipocytes, controls fatty acid uptake, transport, and metabolism in fat cells. We constructed a transgenic mice model that overexpressed the cattle A-FABP gene to investigate the relationship between A-FABP expression and intermuscular fat deposition. There was no significant difference in body weight and ... more

Z.W. Liu; H.L. Fan; X.F. Liu; X.B. Ding; T. Wang; G.N. Sui; G.P. Li; H. Guo

Pages