Research Article

Related GMR Articles

PCR-RFLP; Porcine stress syndrome gene; PSE; Swine

We evaluated the effect of the halothane (HAL) gene on the quality of pork in domestic pigs. Half-carcasses from two different commercial pig (Sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (HALNN) and 69 of which were heterozygous (HALNn) for the halothane gene. The measures included backfat thickness, lean meat percentage, ... more

A.C.P. Silveira; P.F.A. Freitas; A.S.M. César; A.S.M. César; R.C. Antunes; E.C. Guimarães; D.F.A. Batista; L.C. Torido
Cattle; Meat quality traits; MYPN gene; PCR-RFLP; SNP

Myopalladin (MYPN) is a multifunctional protein that maintains sarcomeric integrity and regulates Z-line structure. It is an important candidate gene for meat quality selection through marker-assisted selection. Using PCR-RFLP technology, we discovered a single-nucleotide polymorphism (SNP) (A1795G in exon 9) of the MYPN gene. Allele frequencies of this SNP were investigated and evaluated ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang; B.L. Guo
Avian influenza virus; Chicken; Mx gene; PCR-RFLP; S631N mutation

The Mx (myxovirus resistance) gene codes for a protein with antiviral activity. Non-synonymous G/A polymorphism at position 2032 of chicken Mx cDNA results in a change at amino acid 631 of the Mx protein. This mutation has been shown to affect the antiviral activity of the Mx molecule, although recent studies have not confirmed this effect in response to some influenza ... more

L. Sironi; P. Ramelli; J.L. Williams; P. Mariani
Allelic frequency; Genetic polymorphisms; Hemoglobin S; PCR-RFLP; Sickle cell disease; SNPs

Sickle cell anemia is an affection that causes chronic inflammation, with consequences for vaso-occlusion, oxidative stress and cytokine production. Genetic polymorphisms in markers involved in this process can modulate the inflammatory response, including polymorphisms -308G/A of TNFA (tumor necrosis factor alpha) and -509C/T of TGFB1 (transforming growth factor beta 1), reported to ... more

L.S. Torres; B. Júnior; D.G. Silva; C.L. Lobo; M.A. Ruiz; C.R. Bonini-Domingos
ADAMTS14; Osteoarthritis; PCR-RFLP; rs4747096; Thai population

Osteoarthritis (OA) is the most prevalent form of arthritis in the elderly. This disease is characterized by breakdown and loss of articular cartilage due to genetic, mechanical and environmental factors. Although the pathophysiology of OA is not completely known, several candidate genes have been reported to be associated with OA susceptibility. We assessed the association between ... more

T. Poonpet; S. Honsawek; N. Tammachote; S. Kanitnate; R. Tammachote
Acenocoumarol; APOE; CYP2C19; CYP2C9; Genetic polymorphisms; PCR-RFLP

Oral anticoagulants of the coumarin type have an inconveniently narrow therapeutic window, making their use difficult. In Mexico, genetic variables that participate in the heterogeneity of the therapeutic response remain poorly investigated. With the focus on warfarin, extensive pharmacogenomic studies have been performed, including those on the CYP450 family and APOE. The ... more

J.A. Nastasi-Catanese; J.R. Padilla-Gutiérrez; Y. Valle; F. Ortega-Gutiérrez; M.P. Gallegos-Arreola; L.E. Figuera
Growth traits; IGF-I; PCR-RFLP; Pectoralis major; Synthetic chicken; Thai native chicken

Four Thai synthetic chicken lines (Kaen Thong, Khai Mook Esarn, Soi Nin, and Soi Pet) originated from Thai native and exotic commercial chickens were evaluated for their growth and carcass traits with the purpose of developing a Thai broiler breeding program. Insulin-like growth factor I (IGF-I) gene is known to play an important role in growth, proliferation and differentiation ... more

N. Promwatee; B. Laopaiboon; T. Vongpralub; Y. Phasuk; S. Kunhareang; W. Boonkum; M. Duangjinda
Combined genotype; Goat; PCR-RFLP; SNP

Kisspeptins, the product of the KISS1 gene, play an essential role in the regulation of reproductive functions, acting primarily at the hypothalamic level of the gonadotropic axis. We detected polymorphisms of the goat KISS1 gene in 723 individuals from three goat breeds (Xinong Saanen, Guanzhong, and Boer) by DNA pooling, PCR-RFLP, and DNA sequencing methods. We cloned ... more

X.P. An; P. Han; J.X. Hou; H.B. Zhao; Y. Yan; T. Ma; F. Fang; F.X. Meng; Y.X. Song; J.G. Wang; B.Y. Cao
Clone; Duck; PCR-RFLP; PLIN gene; SNP

The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, ... more

H.L. Zhang; H.J. Fan; X.L. Liu; Y. Wu; S.S. Hou
Centridini; PCR-RFLP; Population variation; Solitary bee

Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola. We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil. Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of the ... more

V.S. Ferreira; C.M.L. Aguiar; E.J.F. Oliveira; M.A. Costa; G.M.M. Santos; J.G. Silva