Research Article

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Proteomics; Two-dimensional gel electrophoresis; Type III brush hair; Yangtze River Delta white goat

The Yangtze River Delta white goat is a goat breed that can produce high quality brush hair (Type III hair) around the world. This study aimed to compare Type III hair and non-Type III hair goat skin tissues using differentially expressed proteins based on 2-dimensional gel electrophoresis technology. The differentially expressed protein spots were analyzed using the PDquest 8.0 software ... more

B. Yang; M.Y. Cai; Y.J. Li; H. Zhang; G.H. Cheng; J.H. Zhang; G.J. Zhang; W.T. Li; D.J. Ji
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Proteomics correspond to the identification and quantitative analysis of proteins expressed in different conditions or life stages of a cell or organism. Methods used in proteomics analysis include mainly chromatography, two-dimensional electrophoresis and mass spectrometry. Data generated in proteomics analysis vary significantly, and to identify a protein it is often necessary to ... more

A.C. Faria-Campos; R.R. Gomes; F.S. Moratelli; H. Rausch-Fernandes; G.R. Franco; S.V.A. Campos
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Human amniotic fluid is of both maternal and fetal origin; it protects the fetus and provides the environment for growth and development of the fetus. We used a proteomics-based approach for targeting and purifying human phosphate binding protein, a member of the DING family of proteins from amniotic fluid, using Blue Sepharose CL-6B, DEAE-Sephacel and gel filtration chroma­tography. The ... more

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Biomarkers; Non-tumoral breast tissue; Proteomics

Breast cancer is a complex and heterogeneous disease. In spite of the advances made in recent decades, a better understanding of the intrinsic mechanisms of this disease is crucial. The development of new biomarkers is absolutely necessary to improve diagnosis and prognosis. Research using the proteomic approach has generated interesting results; however, the complexity of the mammary ... more

G.G. Costa; R. Kaviski; L.E.R. Souza; C.A. Urban; R.S. Lima; I.J. Cavalli; E.M.S.F. Ribeiro
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This study aimed to identify the proteins that are differentially expressed in sera of multiple sclerosis (MS) patients, neuromyelitis optica (NMO) patients, and normal controls by using a two-dimensional gel electrophoresis (2-DE) assay. Serum samples were collected from the 3 groups, and total proteins were isolated and quantified by using the Bradford assay. The 2-DE and silver ... more

S.F. Jiang; Q.Y. Lu; S. Hu; Y. Chen; X.L. Liu; Y. Yang; M.P. Ding
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Chronic intermittent hypoxia; Gender difference; Isobaric tag for relative and absolute quantitation; Obstructive sleep apnea; Proteomics; Vascular injury

Obstructive sleep apnea (OSA) is an independent risk factor for cardiovascular diseases such as systemic arterial hypertension, ischemic heart disease, stroke, heart failure, atrial fibrillation, and cardiac sudden death. The pathogenesis of cardiovascular disease in OSA is thought to be induced primarily by chronic intermittent hypoxia (CIH), a specific pattern of change in oxygenation ... more

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Drug target; Gene medicine; Genomics; Proteomics

Gene medicine is making breakthroughs in health questions that have baffled humanity for centuries. To understand and utilize gene medicine, it is necessary to realize its action against targets at the molecular level. Currently, many methods can be used to discover drug targets; among these, genomic and proteomic methods are the two most important. In this study, we introduced how to ... more

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Cyld knockout; iTRAQ; Kidney disease; Proteomics

The aim of this study was to compare the proteomics pattern of the kidneys from Cyld knockout mice with that from normal mouse kidneys and establish a preliminary understanding of the role of Cyld in the kidney. Proteins from the kidneys of knockout Cyld mice and wild-type mice were extracted, isobaric tags for relative and absolute quantitation (iTRAQ) was ... more

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Abiotic stress; Fatty acids; Proteomics; Transcriptomics; Zea mays

The aim of this study was to characterize maize lines tolerant to cold temperatures during the germination process. Seeds from lines with different levels of tolerance to low temperatures were used; 3 lines were classified as tolerant and 3 as susceptible to low germination temperatures. A field was set up to multiply seeds from selected lines. After the seeds were harvested and ... more

I.C. Silva-Neta; E.V. Pinho; A.D. Veiga; R.G. Pìnho; R.M. Guimarães; F. Caixeta; H.O. Santos; T.L. Marques
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Lipopolysaccharide; Low rumen pH; Proteomics

The aim of this study was to analyze the response of dry goat liver to sub-acute ruminal acidosis induced by a highly concentrated diet. Non-pregnant, non-lactating female Poll-goats (N = 12) were randomly assigned to either a high-concentrate (HG) or a low-concentrate (LG) diet. Low rumen pH was successfully induced with HG (more than 3 h with rumen pH S-transferase was downregulated in ... more

Z. Xie; X. Jiang; P. Ye; Y. Zhang; Y. Ni; S. Zhuang; X. Shen

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