Research Article

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09/10/2013
INH; Invertase gene families; RT-PCR; Tomato

Patterns of gene expression in the different types of sucrose metabolism in the tomato are highly variable and heritable. This genetic variation causes considerable functional differences. We examined the patterns of expression of invertase (Inv) gene families and an invertase inhibitor (INH) gene involved in elongating roots, hypocotyls, and fruit of the tomato (Lycopersicon ... more

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04/02/2013
Apoptosis; Bladder cancer; Gene expression; RT-PCR

We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder ... more

D.F. Barione; F.S.N. Lizarte; P.C. Novais; C.A.M. de Carvalho; F.C.B. Valeri; F.M. Peria; H.F. de Oliveira; D.L. Zanette; W.A. Silva; A.J. Cologna; R.B. Reis; S. Tucci; A.C.P. Martins; D.P.C. Tirapelli; L.F. Tirapelli
01/24/2013
CTAB-LiCl; Gene expression; Nelumbo nucifera; RNA isolation; RT-PCR

Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-quality RNA ... more

Y.J. Zhang; X.Y. Hao; Z.S. Liang; W.D. Ke; H.B. Guo
04/03/2012
Anion exchanger; Boric acid; Gene expression; RT-PCR

Boron is an important micronutrient in plants and animals. The role of boron in living systems includes coordinated regulation of gene expression, growth and proliferation of higher plants and animals. There are several well-defined genes associated with boron transportation and tolerance in plants and these genes show close homology with human anion exchanger genes. Mutation of these ... more

F. Akbas; Z. Aydin
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Drosophila melanogaster; Genome plasticity; hsp22 downregulation; RT-PCR

A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The ... more

L.M. Magdalena; E.C. Coipan; A.F. Vladimirescu; L. Savu; M. Costache; L. Gavrila
03/14/2012
ELISA; IFN-γ; IL-2; Immunopharmacological activity; Piperine; RT-PCR

Piperine, an amide isolated from Piper species (Piperaceae), has been reported to exhibit central nervous system depression, anti-pyretic and anti-inflammatory activity. Immunomodulatory and anti-tumor activity of piperine has been demonstrated in mouse carcinomas. However, there is little information available concerning the effect of piperine on humans. We evaluated the ... more

S. Chuchawankul; N. Khorana; Y. Poovorawan
04/03/2014
Colocalization; Cross-presentation; CTL epitope; Invariant chain; MHC class I; RT-PCR

Cross-presentation (CP) is important for priming T cell responses to many viral, bacterial, and tumor antigens. Here, we designed two Ii mutants, based on evidence that the invariant chain (Ii, also named CD74) binds newly synthesized MHC class I molecules with the class II-associated invariant chain peptide (CLIP) region of Ii, which occupies the peptide-binding groove. Specifically, we ... more

C. Wu; D.G. Zhang; F.F. Chen; X.L. Liu; S.J. Liu; W.Y. Yu
04/03/2014
Easy BlueTM Total RNA Extraction kit; RT-PCR; TLS; Total RNA extraction

Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-BlueTM Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of tissue culture derived ... more

S.H. Habib; H.M. Saud; H. Kausar
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Brain aneurysm; CTA; Nerve growth factor; RT-PCR

We sought to investigate the effect of nerve growth factor (NGF) expression on the formation and prognosis of cerebral aneurysms. Forty-eight cases were selected following a diagnosis of cerebral aneurysm using computed tomography angiography and surgical confirmation. Thirty-four cases of healthy deaths were also chosen. The tissue was tested for NGF expression changes by reverse- ... more

C.D. Wang; D.K. Wang; P.C. Cao; Z.W. Wang; Z. Wang; Y.T. Wang
11/25/2008
desiccation tolerance; high temperature; quantitative trait locus; seeds; Zea mays

Quantitative trait locus (QTL) mapping and identification of traits of agronomic importance is important in the process of molecular marker-assisted selection in breeding programs. The molecular map of maize is well saturated and QTL and simple sequence repeat (SSR) markers have been identified, whereas few markers linked to seed quality traits are included. The present study aimed to identify ... more

K.C.P.C. Salgado; E.V.R. Von Pinho; C.T. Guimarães; R.G. Von Pinho; C.A. Ferreira; V. Andrade

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