Research Article

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05/22/2015
Demographic changes; Genetic heterogeneity; Microsatellites; Mitochondrial sequences; Tayassu pecari

We sequenced the mitochondrial DNA (mtDNA) control region of 59 peccaries (44 white-lipped peccaries, Tayassu pecari, and 15 collared peccaries, Pecari tajacu). We also genotyped 3 DNA microsatellites from 78 white-lipped peccaries representing the 4 putative morphological subspecies (i.e., spiradens, aequatoris, pecari, and albirostris ... more

M. Ruiz-García; M. Pinedo-Castro; K. Luengas-Villamil; C. Vergara; J.A. Rodriguez; J.M. Shostell
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Microsatellites; Polymorphism; Sebastes schlegeli

The Korean rockfish Sebastes schlegeli is a valuable recreational and commercial fish in China, and is cultured in land-based tanks and net cages. Fifteen microsatellite markers were developed for this species, and their polymorphisms were examined in a population. The allele number of the 15 markers ranged from 2 to 13, with an average of 5.933 per locus. The observed and ... more

H.T. Ma; C.F. Jia; J.M. Yang; F. Wang; R. Xue; C.H. Han; H.B. Jiang
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Endangered species; Microsatellites; Next-generation sequencing; Red fox; Vulpes vulpes

The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles ... more

J.N. Yu; C.U. Chung; K.H. Oh; B.K. Lee; C.E. Lim
02/13/2015
Exclusion power; Microsatellites; Parentage analysis; Pig; Single nucleotide polymorphism

Parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. Parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (SNPs) make ... more

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Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control region of DNA extracted from field- ... more

M.I. Espinosa; A. Bertin; F.A. Squeo; A. Cortés; N. Gouin
01/23/2015
Cunninghamia lanceolata; Cupressaceae; Genetic conservation; Microsatellites; Population genetics

As a result of human activities, wild populations of Cunninghamia lanceolata (Cupressaceae) have sharply declined in recent years. The development and implementation of a valid conservation strategy require a clear understanding of the genetic makeup of this species. Eleven polymorphic microsatellite loci were isolated and characterized from samples of 52 individuals from the ... more

Y.X. Li; Z.S. Wang; J.K. Sui; Y.F. Zeng; A.G. Duan; J.G. Zhang
01/15/2015
Microsatellites; Polymorphism; Sinonovacula constricta

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals ... more

H.T. Ma; H.B. Jiang; X.Q. Liu; X.P. Wu; X.M. Wei
12/23/2008
DNA markers; Genetic diversity; Random amplified polymorphic DNA; Sunflower diversity

The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li’s similarity matrix gave values from ... more

M.A. Iqbal; H.A. Sadaqat; I.A. Khan
03/02/2010
Genetic gains; Intrapopulation selection; Selection indexes; Zea mays

We estimated genetic gains for popcorn varieties using selection indexes in a fourth cycle of intrapopulation recurrent selection developed in the campus of the Universidade Estadual do Norte Fluminense. Two hundred full-sib families were obtained from the popcorn population UNB-2U of the third recurrent selection cycle. The progenies were evaluated in a randomized block design with two ... more

A.T.Amaral Júnior; S.P.Freitas Júnior; R.M. Rangel; G.F. Pena; R.M. Ribeiro; R.C. Morais; A.R. Schuelter
08/11/2009
diallel analysis; Genetic divergence; Heterosis; Molecular markers; Popcorn; Zea mays

Diallel analysis was used to obtain information on com­bining ability, heterosis, estimates of genetic distances by random amplified polymorphic DNA (RAPD) and on their correlations with heterosis, for the popcorn varieties RS 20, UNB2, CMS 43, CMS 42, Zélia, UEM J1, UEM M2, Beija-Flor, and Viçosa, which were crossed to obtain all possible combinations, without reciprocals. The genitors ... more

R.E.F. Munhoz; A.J. Prioli; A.T.Amaral Júnior; C.A. Scapim; G.A. Simon

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