Research Article

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DNA markers; Forage legume; Genetic variability

Pityrocarpa moniliformis (Benth.) Luckow and Jobson, commonly known as angico-de-bezerro, is a forage legume that occurs naturally in the Caatinga of northeastern Brazil. This fast growing, vigorous, melliferous tree is well adapted to arid terrains and its branches and leaves possess high nutritional value. However, the scarcity of information regarding genetic variability ... more

F.M.S. Sousa; R.M. Mendes; R.F.M. Mendes; R.B.Araújo Neto; M.P.S.B.C. Nascimento; P.S.C. Lima
07/02/2014
Araceae; Breeding; DNA markers; Genetic diversity; Germplasm; Ornamentals

We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-simple sequence repeat markers were used to estimate the genetic ... more

J.D.Souza Neto; T.C.B. Soares; L.B. Motta; P.D.S. Cabral; J.A. Silva
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Cyclin; Gene duplication; Phylogenetic analysis; Zea mays

Cyclins are primary regulators of the activity of cyclin-dependent kinases and play crucial roles in cell cycle progression in eukaryotes. Although extensive studies have revealed the roles of some cyclins and underlying mechanisms in plants, relatively few cyclins have been functionally analyzed in maize. We identified 59 cyclins in the maize genome, distributed on 10 chromosomes; these ... more

X. Hu; X. Cheng; H. Jiang; S. Zhu; B. Cheng; Y. Xiang
09/13/2013
LEA proteins; qRT-PCR; Zea mays

We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they ... more

T. Andrade; E.V.R. Von Pinho; R.G. Von Pinho; G.E. Oliveira; V. Andrade; J.S. Fernandes
09/30/2007
genetic relationships; Groundnut; Microsatellites; peanut; Simple sequence repeat

The Kayabi Indians who inhabit the Xingu Indigenous Park, located in West Central Brazil, have grown and managed peanuts for a long time. A great number of landraces are being maintained by these tribes and some of this germplasm has morphological traits that exceed the variation described in the taxonomic literature. Here, we analyzed the genetic variability of these landraces using a ... more

F.O. Freitas; M.C. Moretzsohn; J.F.M. Valls
10/05/2007
FISH; Genomic library; Jackknife estimate; Microsatellites; Piracema species; Prochilodus lineatus

We described five novel microsatellite loci for the piracema fish species Prochilodus lineatus (Characiformes), endemic to South America and of extreme importance to both commercial and artisanal fisheries across its occurrence area. A primary, unenriched genomic library was constructed and radioactively screened for repetitive motifs. Positive clones were automatically ... more

G.M. Yazbeck; E. Kalapothakis
05/22/2007
Blastocerus dichotomus; Cervidae; Marsh deer; Microsatellites; Transferability

Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroelectric power station construction and agricultural land expansion. In the present study, we tested 38 microsatellite loci from four Cervidae species: Odocoileus ... more

K.C.E. Leite; R.G. Collevatti; T.R. Menegasso; W.M. Tomas; J.M.B. Duarte
05/31/2007
Genetic variability; Microsatellites; Nuclear molecular markers; Population structure; Wading bird

Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regarding the management and conservation of these populations, we ... more

C.I. Miño; S.N. Del Lama
12/05/2006
CG content; Hyperthermophiles; Microsatellites; Optimum growth temperature; SSRs; Thermophiles

All organisms that have been studied until now have been found to have differential distribution of simple sequence repeats (SSRs), with more SSRs in intergenic than in coding sequences. SSR distribution was investigated in Archaea genomes where complete chromosome sequences of 19 Archaea were analyzed with the program SPUTNIK to find di- to penta-nucleotide repeats. The number of ... more

S. Trivedi
03/22/2007
Brazil; Microsatellites; Mollusca; Mytilidae; Perna perna; Population genetics

Perna perna is the most important cultivated mussel of Santa Catarina, Brazil, sustaining an important economic input for many local families. Natural stocks of P. perna are depleted by the extraction of adults and seeds for consumption and culture. The aim of the present study was to use the microsatellite locus pms-2 to study the variation of the genetic ... more

K.T. Appio; L.I. Weber

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