Research Article

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The repertoire of 4,431 open reading frames (ORFs), eight rRNA operons and 98 tRNA genes of Chromobacterium violaceum must be expressed in a regulated manner for successful adaptation to a wide variety of environmental conditions. To accomplish this feat, the organism relies on protein machineries involved in transcription, RNA processing and translation. Analysis of the C. violaceum ... more

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Chromobacterium violaceum is a Gram-negative β-proteobacterium that inhabits a variety of ecosystems in tropical and subtropical regions, including the water and banks of the Negro River in the Brazilian Amazon. This bacterium has been the subject of extensive study over the last three decades, due to its biotechnological properties, including the characteristic violacein ... more

F.Teixeira Duarte; F.Marques de Carvalho; U.Bezerra e Silva; K.Castanho Scortecci; C.Alfredo Ga Blaha; L.Fassarella Agnez-Lima; S.Regina Bat de Medeiros
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Chromobacterium violaceum is a versatile, Gram-negative β-protebacterium that grows in a variety of ecosystems in tropical and subtropical areas, such as the water and borders of the Negro River, in the Amazon region of Brazil. Although it is a saprophyte and is generally considered non-pathogenic, sporadic cases of human infection have been described, mainly in young children and in ... more

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anthranilate synthase; Chromobacterium violaceum; Homology modeling; tryptophan

Tryptophan is an aromatic amino acid used for protein synthesis and cellular growth. Chromobacterium violaceum ATCC 12472 uses two tryptophan molecules to synthesize violacein, a secondary metabolite of pharmacological interest. The genome analysis of this bacterium revealed that the genes trpA-F and pabA-B encode the enzymes of the tryptophan pathway in which the ... more

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Capsicum annuum; Gene action; Heritability; Meloidogyne incognita; Monogenic inheritance; Resistance

Root-knot nematodes of the genus Meloidogyne are important pathogens affecting vegetable crop production in Brazil and worldwide. The pepper species Capsicum annuum includes both hot and sweet peppers; very little emphasis has been placed on breeding sweet peppers for nematode resistance. We report on the inheritance of resistance to Meloidogyne incognita ( ... more

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Lactuca sativa L.; Lettuce; Meloidogyne javanica; Nematode; Resistance

Resistance to the root-knot nematodes Meloidogyne spp. would be a valuable attribute of lettuce Lactuca sativa L. cultivars grown in tropical regions. The looseleaf lettuce ‘Grand Rapids’ is resistant to both M. incognita and M. javanica. Resistance to M. incognita has a high heritability, under the control of a single gene locus, in which the ... more

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Honey bee comb; Natural comb cell size; Reproduction; Resistance; Varroa destructor

Africanized honey bees (Apis mellifera, Hymenoptera: Apidae) in Brazil are tolerant of infestations with the exotic ectoparasitic mite, Varroa destructor (Mesostigmata: Varroidae), while the European honey bees used in apiculture throughout most of the world are severely affected. Africanized honey bees are normally kept in hives with both naturally built small width ... more

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DNA sequence; Gene-rich region; Molecular marker; Resistance; Yellow rust

Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D ... more

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Actin; Bent DNA; Bombyx mori; Nucleopolyhedrovirus; Resistance

Previous reports demonstrated that actin is necessary for nucleocapsid transport and viral gene expression during nucleopolyhedrovirus infection of Bombyx mori. The first intron of B. mori A3 actin contains a cryptic promoter that drives expression of a rare isoform. We detected differences in the size and nucleotide composition of the first intron of the A3 actin gene ... more

J. Tavares; J.P. Bravo; F. Gimenes; Q.A.L. Neto; A. Fiorini; M.A. Fernandez

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