Short Communication

Related GMR Articles

03/16/2012
ATP1A1; Dairy cow; K+-ATPase; Mastitis; Na+; SNP; Somatic cell score

Mastitis affects the concentrations of potassium and sodium in milk. Since sodium-potassium adenosine triphosphatase (Na+, K+-ATPase) is critical for maintaining the homeostasis of these two ions, and is involved in cell apoptosis and pathogenesis, we presumed that polymorphism of the ATP1A1 gene, which encodes the bovine Na+, K+-ATPase ... more

Y.X. Liu; C.H. Xu; T.Y. Gao; Y. Sun
12/21/2011
Alternative splicing; Dairy cattle; Lactoferrin gene; Mastitis

Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the splice variants of the bLF gene in the mammary ... more

J.M. Huang; Z.Y. Wang; Z.H. Ju; C.F. Wang; Q.L. Li; T. Sun; Q.L. Hou; S.Q. Hang; M.H. Hou; J.F. Zhong
08/26/2011
Dairy cattle; Fertility; Growth hormone; IGF-1; Single nucleotide polymorphism

The somatotrophic axis (GH-IGF) is a key regulator of animal growth and development, affecting performance traits that include milk production, growth rate, body composition, and fertility. The aim of this study was to quantify the association of previously identified SNPs in bovine growth hormone (GH1) and insulin-like growth factor 1 (IGF-1) genes with direct ... more

M.P. Mullen; C.O. Lynch; S.M. Waters; D.J. Howard; P. O’Boyle; D.A. Kenny; F. Buckley; B. Horan; M.G. Diskin
07/30/2013
Dairy cattle; Lactoferrin; Mastitis; TLR4

Mastitis remains a major cattle disease with great global economic implications. Various approaches are currently employed in attempts to improve understanding of mastitis resistance and develop phenotypic markers for use in breeding programs (e.g., somatic cell score), including QTL discovery, wide-genome association studies, and identification of candidate genes related to immune ... more

A.M. Carvajal; P. Huircan; A. Lepori
08/29/2012
Alternative splicing; C4A gene; Dairy cattle; Mastitis; Transcription pattern

The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and ... more

Y. Yang; J.M. Huang; Z.H. Ju; Q.L. Li; L. Zhou; R.L. Li; J.B. Li; F.X. Shi; J.F. Zhong; C.F. Wang
06/15/2012
Covariance function; Dairy cattle; Genetic parameters; Milk yield persistency

With the objective of evaluating measures of milk yield persistency, 27,000 test-day milk yield records from 3362 first lactations of Brazilian Gyr cows that calved between 1990 and 2007 were analyzed with a random regression model. Random, additive genetic and permanent environmental effects were modeled using Legendre polynomials of order 4 and 5, respectively. Residual variance was ... more

R.J. Pereira; R.S. Verneque; P.S. Lopes; M.L. Santana; M.R. Lagrotta; R.A. Torres; A.E.Vercesi Filho; M.A. Machado
12/02/2013
Apoptosis; Heat stress; Hsp90α; Myocardial cells; Rat

To understand the mechanism underlying the sudden animal death caused by acute heart failure during heat stress, the relationships among the heat-induced pathological changes and apoptosis and the variations in the levels of protective Hsp90α and its mRNA in the heat-stressed primary myocardial cells of neonatal rats in vitro were studied by cytopathological observation, ... more

A. Islam; Y.J. Lv; A. Abdelnasir; B. Rehana; Z.J. Liu; M. Zhang; S. Tang; Y.F. Cheng; H.B. Chen; J. Hartung; E.D. Bao
08/20/2013
Heat shock factor-1; Heat shock protein 60; Heat shock protein messenger RNA; Heat stress; Myocardial cells

The mechanisms involved in sudden animal death due to acute heart failure during heat stress are not well understood. We examined the relationship between heat stress-induced variations of protective Hsp60 and expression of its regulatory factor, HSF-1, in heat-stressed primary myocardial cells of neonatal rats in vitro through cardiac enzyme detection, immunoblotting, ... more

R. Buriro; Y.J. Lv; I. Ali; S. Tang; Z.J. Liu; M. Zhang; A. Adem; J. Hartung; E.D. Bao
12/21/2012
Expression; Heat shock protein 110; Heat shock protein 110 messenger RNA; Heat stress; In vitro; Primary myocardial cells of rat

We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and ... more

Z.J. Liu; Y.J. Lv; M. Zhang; Z.H. Yue; S. Tang; A. Islam; B. Rehana; E.D. Bao; J. Hartung
12/18/2014
H9c2 cells; Heat shock protein 47; Heat stress; In vitro; In vivo; Rat heart

The aim of the present study was to identify the correlation between expression of heat shock protein 47 (Hsp47) and stress injury in heat-stressed myocardial cells and to compare variations in Hsp47 expression in rat myocardial cells exposed to different heat stress for varying periods in vitro and in vivo. Exposure to heat stress at 42°C resulted in similar induction ... more

A. Abdelnasir; J.R. Sun; Y.F. Cheng; H.B. Chen; S. Tang; N. Kemper; J. Hartung; E.D. Bao

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