Technical Note

Related GMR Articles

12/19/2011
Cross-amplification; Lycoris radiata; Microsatellite; Molecular marker

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 ... more

S.Q. Xuan; J.Y. Zheng; H. Wang; P. Gao; S.B. Zhou; K. Liu; G.P. Zhu
08/16/2011
Harpadon nehereus; Microsatellite; Molecular marker

The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)15-enriched ... more

T.J. Xu; D.Q. Sun; H.Y. Li; R.X. Wang
02/08/2011
Microsatellite; Molecular marker; Scartelaos viridis

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences contained multiple repeat motifs ( ... more

D.Q. Sun; H.Y. Li; T.J. Xu; R.X. Wang
09/08/2010
Microsatellite; Molecular marker; Mugil cephalus

Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and char­acterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium ... more

T.J. Xu; D.Q. Sun; G. Shi; R.X. Wang
07/13/2010
Microsatellite; Molecular marker; Mytilus coruscus

Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to seven, and the observed and expected ... more

T.J. Xu; Y.N. Sun; Y.T. Yuan; Z. Liao; R.X. Wang
08/06/2012
Microsatellite; Molecular marker; Paris polyphylla var. chinensis; Population genetics

Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)n-enriched ... more

J.Y. Zheng; H. Wang; X.X. Chen; P. Wang; P. Gao; X.N. Li; G.P. Zhu
07/25/2014
Huoyan geese; Microsatellite; Molecular marker

In this study, we isolated microsatellite DNA from the Huoyan goose genome with magnetic beads. As a result, 150 positive clones were identified, and 148 microsatellites were found. Among the 148 microsatellites, 69.6% were perfect, 17.6% were imperfect, and the rest were compound type (12.8%). Twenty microsatellite primers were used to screen 90 individuals from 3 Huoyan goose ... more

Z.Z. Cao; D. Su; Y.Y. Zhao; M. Liu; M. Gao; X.H. Luan
06/20/2007
Canidae; Chrysocyon brachyurus; Cross-amplification; Domestic dog; Microsatellite; Population genetics

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have ... more

D.C. Salim; A.A. Akimoto; C.B. Carvalho; S.F. Oliveira; C.K. Grisolia; J.R. Moreira; M.N. Klautau-Guimarães
07/14/2009
Genetic distance; Genome; Microsatellite; Molecular markers; Population genetics; Triticum aestivum

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity ... more

S. Ijaz; I.A. Khan
12/12/2011
Giant grouper; Isolation; Microsatellite; Population genetics

An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed and expected ... more

S. Yang; L. Wang; Y. Zhang; X.C. Liu; H.R. Lin; Z.N. Meng

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