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Related GMR Articles

11/29/2011
Genetic diversity; Molecular marker; Piper; RAPD; SRAP

Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analysis were applied to 74 individual plants of Piper spp in Hainan Island. The results showed that the SRAP technique may be more informative and more efficient and effective for studying genetic diversity of Piper spp than the RAPD technique. The overall level of genetic ... more

Y. Jiang; J.P. Liu
10/06/2011
ISSR; Molecular marker; RAPD

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 ... more

D.T. Phong; V.T.T. Hien; T.T.V. Thanh; D.V. Tang
01/11/2011
C. equisetifolia subsp incana; Casuarina equisetifolia subsp equisetifolia; Genetic diversity; Molecular marker; Northern coast of Senegal; RAPD

The genetic variation of 70 individual samples of Ca­suarina equisetifolia (L. Johnson) subsp equisetifolia and C. equiseti­folia subsp incana growing along the northern coast of Senegal was analyzed with RAPD markers. Of the 160 primers tested, five were chosen; they generated 1396 reproducible bands and 61 polymorphic bands that were scored. This ... more

A.L. Ndoye; O. Sadio; D. Diouf
07/13/2010
Genetic variability; Heliconia; Molecular marker; Phylogenetic relationships; RAPD; Zingiberales

The family Heliconiaceae contains a single genus, Heliconia, with approximately 180 species of Neotropical origin. This genus was formerly allocated to the family Musaceae, but today forms its own family, in the order Zingiberales. The combination of inverted flowers, a single staminode and drupe fruits is an exclusive characteristic of Heliconia. Heliconias are ... more

L.P. Marouelli; P.W. Inglis; M.A. Ferreira; G.S.C. Buso
12/08/2011
DNA sequence; Gene-rich region; Molecular marker; Resistance; Yellow rust

Analysis of DNA sequence variation among genotypes is useful for differentiation of wheat accessions, selection strategies and genetic development of crop plants. We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the gene-rich regions of wheat chromosomes 1B, 2B, and 7D ... more

E. Cabuk; Y. Aydin; A.A. Uncuoglu
05/04/2010
ISSR; Laboratory optimization; Method evaluation; Molecular marker; PCR

We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize hybrid, one sweet corn, one flint-type corn, ... more

E.C. Oliveira; A.T.Amaral Júnior; L.S.A. Gonçalves; G.F. Pena; S.P.Freitas Júnior; R.M. Ribeiro; M.G. Pereira
12/04/2013
Genetic relationship; Korean Pacific abalone; Molecular marker; Random amplified polymorphic DNA (RAPD); URP primer

Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and ... more

H.S. An; J.Y. Park; J.I. Myeong; C.M. An
10/30/2013
AFLP; Cytoplasmic male sterility; Molecular marker; mtDNA; Wheat

To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta ( ... more

M. Ejaz; Z. Qidi; Z. Gaisheng; W. Qunzhu; Z. Xinbo
10/30/2013
InDel; Insertion-deletion length polymorphism; Molecular marker; Rice (Oryza sativa L.)

In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica ... more

Y.X. Zeng; Z.H. Wen; L.Y. Ma; Z.J. Ji; X.M. Li; C.D. Yang
09/23/2013
Macaw palm; Molecular marker; Oleaginous species; Optimization DNA; PCR amplification; SSR

Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethylammonium bromide ... more

E.C.M. Lanes; C. Nick; K.N. Kuki; R.D. Freitas; S.Y. Motoike

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