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Related GMR Articles

08/03/2010
Cerrado; Erythrinidae; Microsatellite; Shotgun library

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable ... more

S.G.C.A. Gondim; L.V. Resende; R.P.V. Brondani; R.G. Collevatti; N.J. Silva-Júnior; R.R. Pereira; M.P.C. Telles
08/21/2013
Cagaita; Cerrado; Genetic diversity; Microsatellite; Shotgun library

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles ... more

M.P.C. Telles; J.B. Silva; L.V. Resende; R.P. Vianello; L.J. Chaves; T.N. Soares; R.G. Collevatti
11/02/2008
Allozymes; Asteraceae; Campo rupestre; Cerrado; Eremanthus glomerulatus; mtDNA

Tomoplagia reticulata and T. pallens are sibling species that are specialists on Eremanthus glomerulatus. Besides adult terminalia, they show slight morphological differences and distinct geographic distributions. Once, however, they were found sympatrically. Using data from allozyme and mtDNA, we examined patterns of intra- and interspecific genetic structure, and ... more

A.G. Abreu; V.N. Solferini
02/22/2011
Cerrado; Pau-papel; Shotgun library; SSR; STR

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local ... more

M.P.C. Telles; F.P. Peixoto; J.S. Lima; L.V. Resende; R.P. Vianello; M.E.M.T. Walter; R.G. Collevatti
03/01/2011
Cerrado; Dimorphandra mollis; DNA extraction; Metabolic compounds; “Fava d’anta”

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during ... more

P.A. Moreira; D.A. Oliveira
11/27/2013
Alouatta caraya; Assignment test; Brazil; Cerrado; Pedigree; Philopatry

We here investigated the kin structure and pattern of dispersal in the black-and-gold howler monkey (Alouatta caraya, Platyrrhini, Atelidae) based on genotype differences at nine microsatellite loci of 48 individuals from eight social groups along the riparian forest of the Tocantins River, Brazil. The genetic diversity (HE = 0.647) was similar to or higher ... more

R.G. Collevatti; A.C. Souza-Neto; N.J. Silva-Jr.; M.P.C. Telles
09/12/2013
Cerrado; Conservation; Genetic variability; Germplasm; Multivariate techniques

The fragmentation of the original vegetation of the Cerrado biome, caused by the expansion of agricultural areas, mainly in central-west Brazil, calls for an assessment of the native population of this vegetation, especially of the species of interest for domestication and sustainable use. The purpose of this study was to characterize the genetic diversity of 140 gabiroba mother plants ( ... more

E.S. de Assis; E.Fdos Reis; J.F.N. Pinto; L.A.S. Contim; L.A.S. Dias
09/26/2012
Cerrado; Chiroptera; Conservation; Genetic diversity; Neotropical bat; Shotgun genomic library

Glossophaga soricina is a widespread Neotropical nectarivorous bat. We characterized 10 microsatellite loci isolated from a shotgun genomic library. We analyzed tissues from wing membrane of 67 individuals collected from two populations of Central Brazil (Brasília and Alto Paraíso). The number of alleles per locus ranged from 2 to 20, and the observed and expected ... more

M. Oprea; F.P. Peixoto; L.V. Resende; R.G. Collevatti; M.P.C. Telles
06/15/2012
Cerrado; Climatic change; Geographic range shift; Microsatellites

Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and climatic models were combined to model loss of genetic variability in ... more

J.A.F. Diniz-Filho; R.G. Collevatti; L.J. Chaves; T.N. Soares; J.C. Nabout; T.F. Rangel; D.B. Melo; J.S. Lima; M.P.C. Telles
03/22/2012
Cerrado; Dimorphandra mollis; DNA extraction; High-quality DNA; Polysaccharide-free DNA; Secondary metabolites

Dimorphandra mollis (Leguminosae), known as faveiro and fava d’anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications for human circulatory diseases. Knowledge ... more

H.A.V. Souza; L.A.C. Muller; R.L. Brandão; M.B. Lovato

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