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Basic chromosome number; Brachiaria dictyoneura; Cell fusion; Cytokinesis; Interspecific hybridization; Microsporogenesis

Microsporogenesis was analyzed in five accessions of Brachiaria dictyoneura presenting x = 6 as the basic chromosome number. All accessions were tetraploid (2n = 4x = 24) with chromosome pairing in bi-, tri-, and quadrivalents. The recorded meiotic abnormalities were those typical of polyploids, including precocious chromosome migration to the poles, laggard chromosomes, and ... more

C. Risso-Pascotto; M.S. Pagliarini; C.B. Valle
2n gamete; Interspecific hybridization; Meiosis; Pentaploidy; Poaceae

Knowledge about the cytology and reproductive behavior of a species is indispensable for hybridization programs. This is especially true for species belonging to the genus Paspalum, among which apomixis and a wide range of ploidy levels are frequently found. Paspalum conspersum Schrad. is a robust and warm-season perennial bunchgrass native to South America. Previous ... more

L. Janke; F.H.D. Souza; J.F.M. Valls; M.S. Pagliarini
Hexaploidy; Interspecific hybridization; Meiosis; Poaceae; Polyploidy

Cytological investigation revealed complete asynapsis during microsporogenesis in 2 wild accessions of Paspalum jesuiticum collected in distinct Brazilian regions. Both accessions were hexaploid (2n = 6x = 60) and 60 univalents could be counted at diakinesis. In this phase, the majority of meiocytes exhibited univalents with both chromatids. After alignment at the metaphase plate ... more

R.A.Bernardo Filho; A.C.C. Santos; F.H.D. Souza; J.F.M. Valls; M.S. Pagliarini
Bipolaris sorokiniana; Genetic variability; RAPD; Wheat

Isolates of Bipolaris sorokiniana were analyzed by random-amplified polymorphic DNA (RAPD) techniques to determine the amount of intraspecific genetic variability and to study host-pathogen interactions. Ten isolates originated from different regions of Brazil were examined. Plants of the wheat cultivars BR8, BH1146 (original host) and IAC-5 Maringá, classified as resistant ... more

A.T.S. Matsumura; A.M. Prestes; S.T. Van Der Sand; A.M.R. de Oliveira
CTAB; DNA extraction protocol; PVP; RAPD; Sunflower

In order to investigate the mutation characteristics and to further examine the genetic variation of mutant sunflower (Helianthus annuus) obtained in plants grown from seeds exposed to space conditions, only the mature tissues such as leaf and flower could be used for DNA extraction after mutation characteristics were confirmed. The rich contents of polysaccharides, tannins ... more

J.T. Li; D.C. Chen; X.L. Zhang; Z.S. Tang; J. Yang
Genetic diversity; RAPD; Triticum aestivum L.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s ... more

A. Iqbal; A.S. Khan; I.A. Khan; F.S. Awan; A. Ahmad; A.A. Khan
Boll weevil; Isozymes; RAPD; Variability

Twenty-five RAPD loci and 6 isozyme loci were studied to characterize the genetic variability of natural populations of Anthonomus grandis from two agroecosystems of Brazil. The random-amplified polymorphic DNA data disclosed a polymorphism that varied from 52 to 84% and a heterozygosity of 0.189 to 0.347. The index of genetic differentiation (GST) among the six ... more

W.F.S. Martins; C.F.J. Ayres; W.A. Lucena
Ganga; Hooghly; Narmada; PCR; RAPD; Tenualosa ilisha; Yamuna

RAPD was used to delineate the hilsa populations sampled from the Ganga, Yamuna, Hooghly, and Narmada Rivers at six different locations. Six degenerate primers were used to generate the fragment patterns from the samples collected. All primers were highly polymorphic and generated high numbers of amplification products. Nei’s genetic distances were calculated between locations. The ... more

M.P. Brahmane; M.K. Das; M.R. Sinha; V.V. Sugunan; A. Mukherjee; S.N. Singh; S. Prakash; P. Maurye; A. Hajra
Onychophora; Peripatus acacioi; RAPD

RAPD (random amplification of polymorphic DNA) molecular markers can be utilized for analyzing genetic variability in populations for which only a few or no molecular markers are available. They were used in a study of an endangered species, Peripatus acacioi, found in the Tripuí Ecological Station, in Ouro Preto, MG, Brazil. The ecological station was specifically created to ... more

D.Mariele DeLaat; M.Raquel San Carvalho; M.Bernadete Lovato; M.Dolores Po Acedo; C.Graça da Fonseca
Bipolaris sorokiniana; Phytopathogen; RAPD; Variability

Bipolaris sorokiniana is a phytopathogenic fungus causing diseases of cereal crops such as common root rot, the leaf spot disease, seedling blight, and black point of the grain. Random-amplified polymorphic DNA (RAPD) assay was used to investigate the genetic diversity of 20 isolates collected from different cultivars in wheat-producing regions in Brazil. Seventy primers, with random ... more

M.Viviane Go Müller; J.Carlos Germani; S. Van Der Sand