Research Article

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Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

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12S rRNA; Bos gaurus; COI; Cyt b; Malayan gaur; PCR

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine ... more

M.K.A. Rosli; A.S. Zamzuriada; S.M.F. Syed-Shabthar; M.C. Mahani; O. Abas-Mazni; B.M. Md-Zain
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Cereal seeds; DNA extraction; PCR

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves ... more

Z.H. Pervaiz; N.A. Turi; I. Khaliq; M.A. Rabbani; S.A. Malik
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Endometriosis; PCR; Progesterone receptor; PROGINS

We investigated a possible link between endometriosis and polymorphism of the progesterone receptor gene (PROGINS). The endometriosis group consisted of 54 patients with a diagnosis of endometriosis by laparoscopy, and the control group comprised 44 women without endometriosis. Genotypes for PROGINS polymorphisms (A1/A1, A1/A2 and A2/A2) were determined by polymerase chain reaction and ... more

I.R. Costa; R.C.P.C. Silva; A.B. Frare; C.T.X. Silva; B.M. Bordin; S.R. Souza; C.L.Ribeiro Júnior; K.K.V.O. Moura
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Corynebacterium diphtheriae; GenoFrag; Genome plasticity; Horizontal gene transfer; Pathogenicity islands; PCR

Despite the existence of a vaccine against diphtheria, this disease remains endemic and is reemerging in several regions due to many factors, including variations in genes coding for virulence factors. One common feature of virulence factors is their high concentration in pathogenicity islands (PAIs), very unstable regions acquired via horizontal gene transfer, which has lead to the ... more

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03/29/2011
DNA extraction; PCR; Protocol

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single ... more

T.Y. Wang; L. Wang; J.H. Zhang; W.H. Dong
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Brassica napus seeds; Genomic DNA; Lipids; PCR; RAPD; Restriction analysis

A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual biomass (defatted seed residue) for genomic DNA isolation. ... more

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Amazon region; Cervical cancer; HPV; PCR

Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The target region for this study was part of the L1 capsid gene of HPV ... more

M.M. Castro; I.P. Farias; C.M. Borborema-Santos; G. Correia; S. Astolfi-Filho
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False-positive exclusion; PCR; Single-primer PCR correction

Polymerase chain reaction (PCR) technology plays an important role in molecular biology research, but false-positive and nonspecific PCR amplification have plagued many researchers. Currently, research on the optimization of the PCR system focuses on double-primer-based PCR products. This research has shown that PCR amplification based on single-primer binding to the DNA template is an ... more

J. Ma; P.W. Wang; D. Yao; Y.P. Wang; W. Yan; S.C. Guan
11/23/2010
Cladosporium spp; DNA extraction; PCR; rDNA-ITS1

We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted DNA from mycelium mats after manual or electric homogenization. ... more

M.A. Moslem; A.H. Bahkali; K.A. Abd-Elsalam; P.J.G.M. Wit

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