Research Article

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10/03/2011
Broad-complex; Gene expression; In situ hybridization; RACE-PCR; Real-time PCR

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to ... more

A. Buaklin; S. Klinbunga; P. Mensveta
03/09/2011
Gene expression; In situ hybridization; Planarian; Regeneration; STAG

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those ... more

Z.Q. Yuan; B.S. Zhao; J.Y. Zhang
01/23/2015
Cashmere goats; In situ hybridization; Real-time polymerase chain reaction; RORα; RORα expression

The expression of retinoid-acid-related orphan receptor α (RORα) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that RORα ... more

Y.H. Zhao; Z.H. Liu; L. Wang; H.M. Xiao; C.G. Du; Y.J. Zhang; R. Su; J.Q. Li
09/30/2007
Acrididae; Gene mapping; Hsp; In situ hybridization; Schistocerca pallens; Single-copy genes

There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent in situ ... more

T.T. Rieger; S.V. Oliveira-Silva; I.A. Pachêco; B.S. Chagas; J.F. Santos
10/20/2009
Germin; In situ hybridization; RT-PCR; Salt stress; Wheat development

The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of plant development, such as defense, embryonic development and ... more

M. Caliskan
11/29/2011
C-banding; In situ hybridization; Leaf rolling; Triticale; X Triticosecale Wittmack

Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed protein showed that CMH83 was genetically stable. ... more

E.N. Yang; Z.J. Yang; J.F. Zhang; Y.C. Zou; Z.L. Ren
12/02/2013
Expression analysis; Hyriopsis cumingii; In situ hybridization; Laminin receptor precursor; Shell repair

Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability. This study focused on ... more

X.Z. Chang; J.L. Li; Z.Y. Bai; X.L. Li
07/24/2013
Optimized protocol; Pepper; Phytoene desaturase; Tobacco rattle virus; Virus-induced gene silencing

Virus-induced gene silencing is currently a powerful tool for the study of gene function in plants. Here, we optimized the protocol for virus-induced gene silencing, and investigated factors that affect the efficiency of tobacco rattle virus-induced gene silencing in pepper plants. Consequently, an optimal protocol was obtained by the syringe-infiltration method in the leaves of pepper ... more

J.E. Wang; D.W. Li; Z.H. Gong; Y.L. Zhang
07/08/2013
Cross combination; Fertility investigation; Pepper; Rf-linked molecular marker

We studied the efficiency of maintaining and restoring cytoplasmic male sterility (CMS) systems in pepper (Capsicum annuum L.). An Rf-linked molecular marker was employed to analyze the interaction between 6 CMS lines (A), 5 maintainers (B), and 6 restorers (C). Sterility was maintained in the matings of lines 201A x 200B, 203A x 200B, 206A x 200B, 200A x 201B, 206A x ... more

Y. Ma; W. Huang; J.J. Ji; Z.H. Gong; C.C. Yin; S.S. Ahmed; Z.L. Zhao
08/13/2012
High-throughput sequencing; Pepper; Single-nucleotide polymorphism; Single-sequence repeats; Transcriptome

Genetic markers based on single nucleotide polymorphisms (SNPs) are in increasing demand for genome mapping and fingerprinting of breeding populations in crop plants. Recent advances in high-throughput sequencing provide the opportunity for whole-genome resequencing and identification of allelic variants by mapping the reads to a reference genome. However, for many species, such as pepper ... more

M. Nicolaï; C. Pisani; J.P. Bouchet; M. Vuylsteke; A. Palloix

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