Research Article

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Cultivar identification; Molecular markers; Peach; RAPD

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11-mer primers were used to amplify the genotypes; all 95 peach ... more

M.L. Yu; W.Y. Wang; R.J. Ma; Z.J. Shen; J.G. Fang
Divergence; Drimys; Fray Jorge/Talinay; RAPD

The genus Drimys is distributed in Chile from semi-arid zones to sub-Antarctic forests; there are three species of this tree, D. andina, D. confertifolia and D. winteri, the latter with varieties chilensis and winteri. Northern populations are found in small disjunct natural refuges, specifically mountain cloud forests and the bottom of ... more

P. Jara-Arancio; M.R. Carmona; C. Correa; F.A. Squeo; G. Arancio
Altitude; FAME; Genetic distance; RAPD; Vitis vinifera

We examined genetic differences of four Vitis vinifera populations (A, B, C, D) including local Kabarcik cultivar grown along an altitude gradient of 800, 900, 1000, and 1150 m above sea level in the Coruh Valley (800 m: A population; 900 m: B population; 1000 m: C population; 1150 m: D population). Leaf samples were used for both RAPD and fatty acid analysis. A total of 60 ... more

G. Agar; N. Yildirim; S. Ercisli
Landfarm area; Metagenomic; Microbiota representativeness; RAPD

Landfarm soils are employed in industrial and petrochemical residue bioremediation. This process induces selective pressure directed towards microorganisms capable of degrading toxic compounds. Detailed description of taxa in these environments is difficult due to a lack of knowledge of culture conditions required for unknown microorganisms. A metagenomic approach permits identification ... more

J.H. Amorim; R.O. Vidal; G.V. Lacerda-Junior; J.C.T. Dias; M. Brendel; R.P. Rezende; J.C.M. Cascardo
Cultivar discrimination; DNA marker; RAPD; Rosa; SCAR

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. ... more

S. Riaz; B. Sadia; F.S. Awan; I.A. Khan; H.A. Sadaqat; I.A. Khan
Molecular characterization; Pomegranate; Punica granatum; RAPD

The pomegranate is one of the oldest fruits that are traditionally consumed by the local inhabitants of the Coruh Valley, Turkey. In this study, the molecular and morphological characteristics of 19 promising pomegranate genotypes selected from the Coruh Valley were evaluated. For the morphological evaluation, 22 quantitative fruit characteristics were used. For the molecular evaluation, ... more

E. Orhan; S. Ercisli; A. Esitken; M. Sengul
Forage; Genetic diversity; Plant improvement; RAPD; Species-specific markers

Among members of the Fabaceae family, native to the Brazilian Caatinga, the species Poincianella pyramidalis and P. bracteosa exhibit particular potential as forage for cattle, sheep and goats. With the aim of establishing genetic relationships within Poincianella, random amplified polymorphic DNA analysis was performed on eight accessions of P ... more

R.F.M. Mendes; R.B.Araújo Neto; M.P.S.B.C. Nascimento; P.S.C. Lima
Cloning; RAPD; Rose; SCAR; Sequencing

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is ... more

K.M.I. Bashir; F.S. Awan; I.A. Khan; A.I. Khan; M. Usman
Authentication; Dimocarpus longan Lour.; Geographical origin; RAPD

Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, including Sichuan, Hainan, Fujian, ... more

Z.Q. Mei; S.Y. Fu; H.Q. Yu; L.Q. Yang; C.G. Duan; X.Y. Liu; S. Gong; J.J. Fu
Cydonia oblonga Mill.; Genetic diversity; Quince; RAPD

Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 ... more

E. Orhan; G. Nardemir; G. Agar; S. Ercisli