Research Article

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DNA extraction; Leguminosae; RAPD; SRAP; SSR

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and ... more

I.O. Agbagwa; S. Datta; P.G. Patil; P. Singh; N. Nadarajan
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Oenocarpus mapora is an Amazonian palm species commonly used by native populations for food and in folk medicine. We measured genetic variability, using RAPD markers, of material kept in a germplasm bank composed of accessions sampled from the Brazilian Amazon. These included 74 individuals from 23 accessions sampled from 9 localities in three States of the Brazilian Amazon. ... more

E.F. Moura; M.S.P. de Oliveira
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Anthropogenic impact; Genetic diversity; Mytilus galloprovincialis; Pollution; Population structure; RAPD

Despite the great commercial and economic importance of mussels, Mytilus galloprovincialis (Bivalvia, Mollusca) in Greece, little information is available concerning their population genetic structure. We used RAPD markers to examine genetic differentiation and potential impact of aquaculture practices and other anthropogenic activities on the genetic structure of two cultivated ... more

I.A. Giantsis; N. Kravva; A.P. Apostolidis
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Achillea species; Genetic diversity; ISSR; Marker; RAPD

Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, ... more

M. Ebrahimi; M. Farajpour; M. Rahimmalek
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Liriope; Ophiopogon; RAPD; SCAR; Species discrimination

The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to detect ... more

G. Li; Y.J. Park
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DNA fingerprinting; Genetic diversity; Olea europaea; RAPD

A total of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura region of central-western Spain were analyzed using morphological traits and RAPD markers. We focused on three main and 16 minor Spanish olive cultivars that are recognized as native or local to the Extremadura region. The five arbitrary 10-mer primers tested on the olive cultivars gave 67 polymorphic ... more

M.C. Parra-Lobato; F.J. Delgado-Martinez; M.C. Gomez-Jimenez
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Cultivar identification; Molecular markers; RAPD; Sweet orange

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ... more

X. Sun; Q. Mu; D. Jiang; C. Wang; X.C. Wang; J.G. Fang
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AFLP; Ficus carica; Genetic variability; RAPD

The fig (Ficus carica L.) is a fruit tree of great world importance and, therefore, the genetic improvement becomes an important field of research for better crops, being necessary to gather information on this species, mainly regarding its genetic variability so that appropriate propagation projects and management are made. The improvement programs of fig trees using ... more

M.G.F. Rodrigues; A.B.G. Martins; J.A. Desidério; B.W. Bertoni; M.C. Alves
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ISSR; RAPD; Sequence characterized amplification region; Thinopyrum elongatum

Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We used 36 RAPD primers and 33 ISSR primers to ... more

G.H. Xu; W.Y. Su; Y.J. Shu; W.W. Cong; L. Wu; C.H. Guo
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16S rRNA; Diversity; ERIC-PCR; High-throughput screening; Proteobacteria; RAPD

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia ... more

L.H. Lee; Y.K. Cheah; A.M.Nurul Syakima; M.S. Shiran; Y.L. Tang; H.P. Lin; K. Hong

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