Research Article

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09/21/2011
Cattle; CYP21; Genotype-phenotype association; IGF2; SNPs

We analyzed two single nucleotide polymorphisms (SNPs) of the IGF2 and CYP21 genes in Nellore cattle participating in the Brazilian Animal Breeding Program. The SNPs were found in exon 6 of the IGF2 (insulin-like growth factor 2) gene (RFLP/MboII) as well as in the promoter region of the CYP21 (steroid 21-hydroxylase) gene (RFLP/HpaII) ... more

M. da Silva; A.F.L. Rios; E.S. Ramos; R.B. Lôbo; H.N. Oliveira; M.A.R. de Freitas
09/09/2011
ACOX1 gene; Cattle; PCR-SSCP; SNP; Ultrasound traits

Acyl-coenzyme A oxidase 1 (ACOX1) is the first enzyme in peroxisomal fatty acid β-oxidation; it is rate-limiting and plays a key role in fatty acid metabolism and fat deposition. ACOX1 is an important candidate gene for meat quality selection through marker-assisted selection. Genomic structural analysis showed that bovine ACOX1 shares 86% identity with human ACOX1. Using PCR-SSCP ... more

Y. Jiao; L.S. Zan; Y.F. Liu; H.B. Wang
04/17/2013
Body weight; Cattle; Dapper1 gene; F-PCR-RFLP; Haplotype; SNPs

The Dapper1 protein plays important role in multiple developmental processes via negative modulation of the Wnt signaling pathway. We detected variations in Dapper1 in 1185 individuals from 5 Chinese cattle breeds and determined their effects on bovine body weight. Two silent mutations (g.8344C>T and g.8428C>T) in exon 6 along with two substitutions (g.10513A>G and g. ... more

J. Wang; C. Wang; Y. Gao; X.Y. Lan; C.Z. Lei; J.Q. Wang; H. Chen
08/29/2012
ANAPC13; Body measurement traits; Cattle; PCR-SSCP; Polymorphism

Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. There has been some indication that ANAPC13 influences adult height. We used PCR-SSCP and DNA sequencing technology to identify polymorphisms in the ANAPC13 gene. A polymorphism in intron 1 (A > G at base 17) was identified and an ... more

B.J. Jiang; X.L. Zhan; C.Z. Fu; H.B. Wang; G. Cheng; L.S. Zan
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Goat; Growth traits; Myostatin gene (MSTN); PCR-SSCP; Polymorphism

Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for ... more

Z.J. Zhang; Y.H. Ling; L.J. Wang; Y.F. Hang; X.F. Guo; Y.H. Zhang; J.P. Ding; X.R. Zhang
08/11/2006
BoLA; Bovine; Candidate genes; Cattle; Molecular markers; Tick resistance

Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to many chemicals. More recently, vaccines have been used in ... more

M.L. Martinez; M.A. Machado; C.S. Nascimento; M.V.G.B. Silva; R.L. Teodoro; J. Furlong; M.C.A. Prata; A.L. Campos; M.F.M. Guimarães; A.L.S. Azevedo; M.F.A. Pires; R.S. Verneque
08/07/2006
Candidate genes; Cattle; DGAT1; PCR-RFLP; Zebu

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection ... more

G.A. Lacorte; M.A. Machado; M.L. Martinez; A.L. Campos; R.P. Maciel; R.S. Verneque; R.L. Teodoro; M.G.C.D. Peixoto; M.R.S. Carvalho; C.G. Fonseca
12/27/2005
Cattle; Cell isolation; Multiplex-PCR; Nuclear transfer; Transgene detection

Transgenesis in cattle has provided numerous opportunities for livestock production. The development of nuclear transfer (NT) technology has improved the production of transgenic livestock. However, the isolation of pure colonies from a single transfection event remains laborious and can be a constraint in the production of transgenic livestock. We used 96-well cell culture plates to ... more

E.O. Melo; R.V. Sousa; L.T. Iguma; L.T. Iguma; M.M. Franco; E.L. Rech; E.L. Rech; R. Rumpf
09/29/2009
Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

E. Groeneveld; Bv.d. Westhuizen; A. Maiwashe; F. Voordewind; J.B.S. Ferraz
01/27/2009
Cattle; Epigenetics; Genomic imprinting; IGF2; IGF2R; Nuclear transfer

Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by ... more

F. Perecin; S.C. Méo; W. Yamazaki; C.R. Ferreira; G.K.F. Merighe; F.V. Meirelles; J.M. Garcia

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