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Japanese Spanish mackerel; Microsatellite loci; Population structure; Scomberomorus niphonius

We isolated and characterized 21 polymorphic microsatellite loci in Japanese Spanish mackerel (Scomberomorus niphonius) using a (GT)13-enriched genomic library. Forty individuals were collected from Qingdao, China. We found 3 to 24 alleles per locus, with a mean of 8.8. The observed and expected heterozygosities ranged from 0.263 to 0.975 and from 0.385 to 0.946, with ... more

L. Lin; L. Zhu; S.F. Liu; Q.S. Tang; Y.Q. Su; Z.M. Zhuang
Black rockfish; Genetic structure; Hatchery strain; Microsatellite loci; Sebastes inermis; Wild population

The population structure of the black rockfish, Sebastes inermis (Sebastidae), was estimated using 10 microsatellite loci developed for S. schlegeli on samples of 174 individuals collected from three wild and three hatchery populations in Korea. Reduced genetic variation was detected in hatchery strains [overall number of alleles (NA) = 8.07; allelic ... more

H.S. An; E.M. Kim; J.H. Lee; J.K. Noh; C.M. An; S.J. Yoon; K.D. Park; J.I. Myeong
Genetic structure; Korean rockfish; Microsatellite loci; Sebastes schlegeli

The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the northern coastal waters of the Yellow Sea. ... more

C.C. Bai; S.F. Liu; Z.M. Zhuang; L. Lin; H. Wang; Y.Q. Chang
Artificial reproduction; Black rockfish; Genetic variability; Microsatellite loci; Mitochondrial DNA; Sebastes inermis

The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a mitochondrial (mt) ... more

H.S. An; J.W. Lee; J.Y. Park; J.I. Myeong; C.M. An
Genetic variability; Korean threadsail filefish; Microsatellite loci; Next-generation sequencing; Stephanolepis cirrhifer

The threadsail filefish, Stephanolepis cirrhifer (Monacanthidae), is found mainly in the western Pacific. It is intensively caught in Korea and is a highly appreciated seafood delicacy. Consequently, the natural population of this species has drastically decreased, despite introductions from hatcheries. To provide information necessary for its conservation and management, we ... more

C.M. An; H.S. An; J.W. Lee; S.W. Hong
Atrina pectinata; Microsatellite loci; Polymorphic

In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied ... more

B. Wu; L.J. Bai; A.G. Yang; L.Q. Zhou; Z.H. Liu
Amazon basin; Apapa; Enriched genomic library; Microsatellite loci; Population analysis

The Amazon pellona is one of the few species of Pristigasteridae with recognized commercial value in the Amazon. We isolated 24 and characterized 8 microsatellite loci for this species. The number of alleles ranges from 2-8 per locus. Observed heterozygosities ranged from 0.052-0.823, while expected heterozygosities from 0.052-0.836. These 8 microsatellites are potentially valuable tools ... more

A.M. Ximenes; E.J. Hernández-Ruz; V.N. Machado; L.R.R. Rodrigues; G.C.S. Ritter; I.P. Farias
Gene flow; Genetic variability; Mating strategies; Nellore; Population structure

Parameters based on the probability of gene origin were used to describe genetic variability in three reproductive groups from the Breeding Program for Nellore Cattle (PMGRN). The three reproductive populations (cows in reproductive age, bulls from artificial insemination centers and young bulls in progeny test) generated medium to low values. The effective number of founders (N ... more

P.A. Vozzi; C.R. Marcondes; L.A.F. Bezerra; R.B. Lôbo
Genetic variability; Microsatellites; Nuclear molecular markers; Population structure; Wading bird

Roseate Spoonbills (Platalea ajaja, Linnaeus) are wading birds present in two of the most important Brazilian wetlands: the Pantanal wetlands and Rio Grande do Sul marshes. Natural populations of these species have not been previously studied with variable nuclear molecular markers. In order to support decision making regarding the management and conservation of these populations, we ... more

C.I. Miño; S.N. Del Lama
Coalescent theory; Pairwise differences; Population structure; Refugia

The climatic fluctuations of the Quaternary have influenced the distribution of numerous plant and animal species. Several species suffer population reduction and fragmentation, becoming restricted to refugia during glacial periods and expanding again during interglacials. The reduction in population size may reduce the effective population size, mean coalescence time and genetic ... more

F.F. Jesus; J.F. Wilkins; V.N. Solferini; J. Wakeley