Research Article

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Arabidopsis; Gene expression; Glucose; Transcriptome

Sugars acting as fuel energy or as signaling molecules play important roles in plant growth and development. Although sugars associated with early seedling development have been analyzed in detail, few studies have examined the effect of sugar on genome-wide gene transcription. To analyze the role of glucose on the genomic level, we examined the response of seedlings to 5% glucose using ... more

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Female sterile homeotic (fsh); Gene cloning; Gene expression; Macrobrachium nipponense

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its ... more

W.Y. Zhang; S.F. Jiang; Y.W. Xiong; H.T. Fu; H. Qiao; S.M. Sun; Y.S. Gong; S.B. Jin
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Chicken; Cloning; Gene expression; Newcastle disease virus; Tumor necrosis factor receptor-associated factor 3

Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (chTRAF3) and detected ... more

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Beta-casein; Epithelial cell culture; Gene expression; Mammary gland

Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established primary cell ... more

J. Ogorevc; P. Dovč
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Gene expression; Hu-sheep; MSTN; Muscle fiber development; MyoG; YAP1

The aim of the current study was to investigate the effects of Yes-associated protein 1 (YAP1) gene expression after birth on the development of muscle and the relationship between YAP1 and myostatin (MSTN) and myogenin (MyoG). Reverse transcription polymerase chain reaction was used to analyze the trends in YAP1, MSTN, and MyoG ... more

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Gene expression; Gene structure; Nilaparvata lugens; NlABCG; Tissue distribution

The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. In insects, ABC transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. In this study, the Nilaparvata lugens Stal (Hemiptera: ... more

W.J. Zha; S.H. Li; L. Zhou; Z.J. Chen; K. Liu; G.C. Yang; G. Hu; G.C. He; A.Q. You
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Chinese cabbage; Gene expression; Hsf; Interaction network; Phylogenetic analysis

Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetable crops grown worldwide, and various methods exist for selection, propagation, and cultivation. The entire Chinese cabbage genome has been sequenced, and the heat shock transcription factor family (Hsfs) has been found to play a central role in plant growth and development and in the ... more

X.Y. Huang; P. Tao; B.Y. Li; W.H. Wang; Z.C. Yue; J.L. Lei; X.M. Zhong
03/27/2015
Gene expression; Microarray; Network; Protein-protein interaction; Renal cell carcinoma

The aims of this study were to identify the common gene signatures of clear cell renal cell carcinoma (CCRCC), and to expand the respective protein-protein interaction networks associated with CCRCC regulation. For the latter, we utilized multiple gene expression data sets from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO), with which we could ... more

H.J. Zhang; Z.Q. Sun; W.Q. Qian; L. Sheng
03/06/2015
endosperm; Gene expression; Hormone; Maize; Sugar

Starch is the major storage product in the endosperm of cereals. Its synthesis is closely related to sucrose metabolism. In our previous study, we found that the expression of most of the genes involved in starch synthesis might be regulated by sugars and hormones in the maize endosperm. However, little is known regarding the transcriptional regulation of genes involved in sucrose ... more

X.D. Ren; H.M. Liu; Y.H. Liu; Y.F. Hu; J.J. Zhang; Y.B. Huang
03/06/2015
Gene expression; Growth stages; Mitochondrial DNA copy number; Pig

Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult. Observable differences in mtDNA ... more

Y.M. Xie; L. Jin; X.J. Chen; M.N. He; Y. Wang; R. Liu; M.Z. Li; X.W. Li

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