Research Article

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DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was ... more

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Ganga; Hooghly; Narmada; PCR; RAPD; Tenualosa ilisha; Yamuna

RAPD was used to delineate the hilsa populations sampled from the Ganga, Yamuna, Hooghly, and Narmada Rivers at six different locations. Six degenerate primers were used to generate the fragment patterns from the samples collected. All primers were highly polymorphic and generated high numbers of amplification products. Nei’s genetic distances were calculated between locations. The ... more

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Amplification success; Barcoding; Birds; Cytochrome oxidase 1; Mammals; Mini-barcode; PCR; Reptiles

In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single ... more

I.A. Arif; H.A. Khan; A. Sadoon; M. Shobrak
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BLV testing; Bovine leukemia virus; Cattle; Leukosis; Native Iranian and Russian breeds; PCR

Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

M.R. Mohammadabadi; M. Soflaei; H. Mostafavi; M. Honarmand
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12S rRNA; Bos gaurus; COI; Cyt b; Malayan gaur; PCR

PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various trials and combinations were tested to determine ... more

M.K.A. Rosli; A.S. Zamzuriada; S.M.F. Syed-Shabthar; M.C. Mahani; O. Abas-Mazni; B.M. Md-Zain
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Cereal seeds; DNA extraction; PCR

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves ... more

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Corynebacterium diphtheriae; GenoFrag; Genome plasticity; Horizontal gene transfer; Pathogenicity islands; PCR

Despite the existence of a vaccine against diphtheria, this disease remains endemic and is reemerging in several regions due to many factors, including variations in genes coding for virulence factors. One common feature of virulence factors is their high concentration in pathogenicity islands (PAIs), very unstable regions acquired via horizontal gene transfer, which has lead to the ... more

S.C. Soares; F.A. Dorella; L.G.C. Pacheco; R. Hirata; A.L. Mattos-Guaraldi; V. Azevedo; A. Miyoshi
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DNA extraction; PCR; Protocol

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single ... more

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Brassica napus seeds; Genomic DNA; Lipids; PCR; RAPD; Restriction analysis

A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual biomass (defatted seed residue) for genomic DNA isolation. ... more

M. Sadia; M.A. Rabbani; S. Hameed; S.R. Pearce; S.A. Malik
02/08/2011
Amazon region; Cervical cancer; HPV; PCR

Infection by human papillomavirus (HPV) is one of the primary causes of mortality by cancer in northern Brazil. Sexually active women from Manaus, Amazonas, without cytological alterations and women with pre-malignant and malignant cytological alterations were examined for HPV virus, identified via PCR and sequencing. The target region for this study was part of the L1 capsid gene of HPV ... more

M.M. Castro; I.P. Farias; C.M. Borborema-Santos; G. Correia; S. Astolfi-Filho

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