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Related GMR Articles

Cervus unicolor; Conservation; Microsatellite; PCR-based isolation of microsatellite arrays; RAPD-PCR enrichment

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg ... more

D.Y. Lin; T.Y. Chiang; C.C. Huang; H.D. Lin; S.J. Tzeng; S.R. Kang; H.M. Sung; M.C. Wu
Conservation; Holothuria scabra; Microsatellite; Sea cucumber

The sea cucumber Holothuria scabra is an endangered species. In this study, nine new polymorphic microsatellite loci were developed and tested in 30 individuals. The number of alleles ranged from 2 to 5, and the observed and expected heterozygosities ranged from 0.1200 to 0.7391 and from 0.2408 to 0.5983, respectively. No loci significantly deviated from the Hardy-Weinberg ... more

Z.B. Li; G. Dai; J.B. Shangguan; Y.F. Ning; Y.Y. Li; R.B. Chen; Y.S. Huang; Y. Yuan
Amazona aestiva; bottleneck; Conservation; Genetic structure; Microsatellite

The blue-fronted Amazon (Amazona aestiva) is a widely distributed Neotropical parrot and one of the most captured parrots in nature to supply the illegal trade of wild animals. The objectives of the present study were to analyze the genetic structure of A. aestiva to identify management units and support conservation planning and to verified if A. aestiva populations ... more

K.C.E. Leite; G.H.F. Seixas; I. Berkunsky; R.G. Collevatti; R. Caparroz
bees; Conservation; Melipona; Microsatellite; Transferability

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Melipona has the ... more

M.V.C. Viana; E.A. Miranda; A.K. de Francisco; C.A.L. Carvalho; A.M. Waldschmidt
Chinese indigenous buffalo; Conservation; Hybrid offspring; Microsatellite

Chinese native buffaloes have faced the threat of extinction, along with an increase in crossbreeding with domesticated river buffaloes; consequently, conservation of local buffalo genetic resources has become a priority. A Chinese native breed, Jianghan, is often crossed intentionally and unintentionally with imported breeds from India and Pakistan, Murrah, and Nili-Ravi. A total of 128 ... more

W.C. Yang; K.Q. Tang; J. Mei; W.B. Zeng; L.G. Yang
Conservation; Greek black pig; Microsatellite

Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays, the worldwide distribution of pigs is dominated by a few breeds, tending towards a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. The Greek black pig, a breed raised locally and known for the high quality of its meat for cured ... more

S. Michailidou; A. Kalivas; I. Ganopoulos; E. Stea; G. Michailidis; A. Tsaftaris; A. Argiriou
Common dormouse; Conservation; Genetic structure; Microsatellite; Movement

Movement is an important life history trait that can have an impact on local adaptation, and other evolutionary phenomena. We used a combination of nestbox survey data and genetic techniques (genotyping at 10 microsatellite loci) to quantify patterns of movement in common dormice Muscardinus avellanarius at two distinct sites in the UK: 1) Bontuchel (a natural population) and 2) ... more

D.M. Naim; S. Telfer; S. Tatman; S. Bird; S.J. Kemp; P.C. Watts
Canidae; Chrysocyon brachyurus; Cross-amplification; Domestic dog; Microsatellite; Population genetics

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have ... more

D.C. Salim; A.A. Akimoto; C.B. Carvalho; S.F. Oliveira; C.K. Grisolia; J.R. Moreira; M.N. Klautau-Guimarães
Genetic distance; Genome; Microsatellite; Molecular markers; Population genetics; Triticum aestivum

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity ... more

S. Ijaz; I.A. Khan
Giant grouper; Isolation; Microsatellite; Population genetics

An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed and expected ... more

S. Yang; L. Wang; Y. Zhang; X.C. Liu; H.R. Lin; Z.N. Meng