Methodology

Related GMR Articles

07/25/2006
Brahman; Carcass; DNA; Genetic marker; Growth; Growth hormone

Associations of DNA polymorphisms in growth hormone (GH) relative to growth and carcass characteristics in growing Brahman steers (N = 324 from 68 sires) were evaluated. Polymorphisms were an Msp-I RFLP and a leucine/valine SNP in the GH gene as well as a Hinf-I RFLP and a histidine/arginine SNP in transcriptional regulators of the GH gene, Pit-1 and Prop-1. Genotypic frequencies of ... more

V.R. Beauchemin; M.G. Thomas; D.E. Franke; G.A. Silver
09/06/2005
Composite beef cattle; DNA; Genetic characterization; RFLP

A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to determine the structure and the genetic variability of this hybrid ... more

E.Perez Marson; J.Bento Ster Ferraz; F.Vieira Meirelles; J.César de Balieiro; J.Pereira Eler; L.Gustavo Gi Figueiredo; G.Barreto Mourão
04/13/2005
DNA; Minisequencing; Multiplex; Polymorphisms; Single nucleotide polymorphisms

Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research laboratories. Minisequencing meets the latter requirements. It is ... more

C.M.B. Carvalho; S.D.J. Pena
08/03/2010
DNA; DYS464; Microsatellites; Population genetics; Worldwide populations; Y-chromosome

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 repeats. One hundred and seventy-five ... more

F.S.G. Kehdy; S.D.J. Pena
08/17/2010
DNA; Equine; Lung; PBMC; Protocol

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different protocols. Protocol A consisted ... more

E.M. Santos; J.F.R. Paula; P.M.C. Motta; M.B. Heinemann; R.C. Leite; J.P.A. Haddad; H.L. Del Puerto; J.K.P. Reis
03/30/2010
DNA; Indels; Insertion-deletion polymorphisms; Paternity testing; Single nucleotide polymorphisms

We developed a panel of 40 multiplexed short insertion-deletion (indel) polymorphic loci with widespread chromosomal locations and allele frequencies close to 0.50 in the European population. We genotyped these markers in 360 unrelated self-classified White Brazilians and 50 mother-child-probable father trios with proven paternity. The average heterozygosity (gene diversity) per locus was ... more

J.R. Pimenta; S.D.J. Pena
03/09/2010
DNA; Essential oil; PCR amplification; RAPDs; Tagetes minuta

Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be a medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties. Tagetes minuta characteristically contains high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA, causing ... more

I. Shahzadi; R. Ahmed; A. Hassan; M.M. Shah
07/30/2013
Cheilinus undulatus; DNA; Humphead wrasse; Microsatellite

Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed and expected heterozygosity ... more

J. Hu; X.P. Zhu; J. Luo; S.W. Yin; Y.H. Peng; Y.L. Hu; F. Zhu
03/11/2013
DNA; Germination; Polyvinylpyrrolidone; Sodium dodecyl sulfate; Vigor

Genomic tools for watermelon breeding are becoming increasingly available. A high throughput genotyping system would facilitate the use of DNA markers in marker-assisted selection. DNA extraction from leaf material requires prior seed germination and is often time-consuming and cost prohibitive. In an effort to develop a more efficient system, watermelon seeds of several genotypes and ... more

G. Meru; D. McDowell; V. Waters; A. Seibel; J. Davis; C. McGregor
04/10/2015
DNA; Forensic DNA; Indels; Insertion-deletion polymorphisms

We have previously developed a panel of 40 insertion-deletion (INDEL) human DNA polymorphisms that was proven to ad­equately cover the span of global human genetic diversity. The panel was found to have very low matching probabilities with respect to both the global and Brazilian populations. To optimize the panel for application with degraded DNA samples, which are commonly encountered ... more

V.R.D. Santos; H.B. Pena; S.D.J. Pena

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