Research Article

Related GMR Articles

03/31/2003
Double-stranded RNA (dsRNA); Gene expression; RNA interference; Trypanosoma

Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramming of genetic expression. Using primarily Trypanosoma ... more

S.M.R. Teixeira; W.D. da Rocha
09/04/2013
PTHrP; Recombinant adenovirus; RNA interference

Parathyroid hormone-related protein (PTHrP) is a protein member of the parathyroid hormone family that regulates the dynamic balance between blood and bone calcium during lactation. However, the mechanism of its regulation is not very clear. In order to establish a framework for further functional studies of the PTHrP gene in goat mammary gland epithelial cells during ... more

H.L. Zheng; X.M. Liu; Q. Yu; R.F. Xing; Z.Y. Yang; J. Luo
01/04/2013
Amylose content; RBE3 gene; Rice; RNA interference

Amylose and amylopectin are the 2 major components of plant storage starch. The rice starch branching enzyme (RBE) plays an important role in the starch components of rice. In the present study, we selected a specific 195-bp segment from the RBE3 gene to construct hairpin DNA, which was driven by an endosperm-specific high molecular weight glutenin promoter to regulate the ... more

H.Y. Jiang; J. Zhang; J.M. Wang; M. Xia; S.W. Zhu; B.J. Cheng
08/28/2014
Gonadotropin-releasing hormone; Neural tissue-specific epidermal growth factor-like repeat domaincontaining protein; Puberty; RNA interference

Neural tissue-specific epidermal growth factor-like repeat domain-containing protein (NELL2) was previously found to play an important role in nerve growth, neural differentiation, neural elasticity, synaptic transport, and vesicle release. In this study, we examined the effect of NELL2 on gonadotropin-releasing hormone (GnRH) neurons and the initiation of puberty in female rats. We ... more

S.S. Zhou; P. Li
08/07/2014
Adipocyte; Chicken; Leptin receptor; Lipid metabolism; RNA interference

In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P < 0.01). After the knockdown of the leptin receptor, the expression levels of certain genes decreased significantly; specifically, peroxisome ... more

A.X. Huang; J.J. Li; Y. Tian; J.D. Shen; Z.R. Tao; G.Q. Li; L.Z. Lu; Y. Fu; T.X. Wu
07/07/2014
Cervical cancer; Radiosensitivity; RNA interference; SiHa cells; Vascular endothelial growth factor

This study aimed to investigate the inhibition of vascular endothelial growth factor (VEGF) by small hairpin RNA (shRNA) interference combined with radiotherapy on the growth of cervical cancer SiHa cell xenografts in nude mice. The effective pVEGF-shRNA plasmid was screened by reverse transcription-polymerase chain reaction (RT-PCR), and the cell apoptosis rate was estimated by flow ... more

L. Qi; L.N. Xing; X. Wei; S.G. Song
04/10/2015
Alpha-fetoprotein; Hepatocellular carcinoma; RNA interference; Survivin

We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect cell proliferation activity ... more

Z.L. Fang; N. Fang; X.N. Han; G. Huang; X.J. Fu; G.S. Xie; N.R. Wang; J.P. Xiong
04/10/2015
AP-1; Apoptosis; MC3T3-E1 osteoblast; p38MAPK; RNA interference

We investigated the effect of p38MAPK/AP-1 (activator protein-1) signaling on the apoptosis of osteoblasts induced by high glucose. A lentivirus vector of small hairpin RNA (shRNA) targeting p38MAPK was constructed in vitro. Osteoblasts MC3T3-E1 cultured in vitro were treated with vehicle, high glucose, p38MAPK-shRNA transfection, p38MAPK inhibitor, and unrelated shRNA ... more

Z.P. Feng; H.C. Deng; R. Jiang; J. Du; D.Y. Cheng
01/16/2015
Construction of recombinant adenovirus vector; Gene transfection; RNA interference; Vector

The aim of this study was to construct the recombinant adenovirus carrying human TIMP-1shRNA gene expression system for preliminary identification to lay the foundation for the further study of gene therapy. Using the Adeno-XTM system, the recombinant adenovirus plasmid pAdeno-XTM green fluorescent protein (GFP)-tissue inhibitor of metalloprotease (TIMP)-1 small ... more

Y.L. Sun; H. Xie; H.L. Lin; Q. Feng; Y. Liu