Research Article

Related GMR Articles

Body measurement traits; DKK4 gene; PCR-SSCP; Qingchuan cattle; SNPs

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of bovine Dickkopf homolog 4 (DKK4) are associated with body measurement traits in Qinchuan cattle. By using PCR-SSCP technology and DNA sequencing, we discovered 5 DKK4 SNPs in Qingchuan cattle, including -65G>A and -77G>T in the 5ꞌ-untranslated region, 1532C>G and 1533T>C ... more

J.B. Gao; Y.K. Li; N. Yang; X.H. Ma; C. Adoligbe; B.J. Jiang; C.Z. Fu; G. Cheng; L.S. Zan
Growth hormone gene; PCR-SSCP; Wistar-Imamichi rat

We successfully cloned the Wistar-Imamichi (WI) rat growth hormone gene (GenBank accession: GQ890681), which contained 5 exons and 4 introns. Using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique, a novel missense substitution single nucleotide polymorphism was identified and tested for Hardy-Weinberg equilibrium with the χ2 test. This ... more

M.Q. Liu; J.G. Wang; X.L. Li
Goat; Growth traits; Myostatin gene (MSTN); PCR-SSCP; Polymorphism

Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigated as a candidate marker for ... more

Z.J. Zhang; Y.H. Ling; L.J. Wang; Y.F. Hang; X.F. Guo; Y.H. Zhang; J.P. Ding; X.R. Zhang
ARMS-PCR; Genotyping; mutations; PCR-SSCP; PRNP alleles; Sheep

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from ... more

C.L. Zhao; R. Wu; L. Liu; F.D. Li; X.L. Zhang; C. Wang; F. Wang; X.L. Diao; H.W. Guan; X. Wang; L. Zhou
GFI1B gene; Growth trait; Indigenous Chinese goats; PCR-RFLP; PCR-SSCP

This study aimed to investigate polymorphisms of the eighth exon in the GFI1B gene among three indigenous Chinese goat breeds (QianBei Ma goats, GuiZhou white goats, and GuiZhou black goats). Furthermore, association analysis was conducted between these polymorphisms and growth traits. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), direct DNA ... more

H.F. Cai; Z. Chen; W.X. Luo
Beef cattle; Bos indicus; Leptin; Molecular markers; Ultrasound traits

Given the important role of leptin in metabolism, we looked for a possible association of leptin and leptin receptor polymorphisms with carcass and growth traits in Nellore cattle. We examined associations of leptin and leptin receptor SNPs with ultrasound carcass (longissimus dorsi muscle area (ribeye area), backfat thickness and rump fat thickness and growth traits (weaning weight ... more

R.C.G. da Silva; J.B.S. Ferraz; F.V. Meirelles; J.P. Eler; J.C.C. Balieiro; D.C. Cucco; E.C. Mattos; F.M. Rezende; S.L. Silva
Brangus; Carcass; DNA; Growth; Growth hormone; SNP

Sequence polymorphisms in the growth hormone (GH) gene and its transcriptional regulators, Pit-1 and Prop-1, were evaluated for associations with growth and carcass traits in two populations of Brangus bulls Chihuahuan Desert Rangeland Research Center (CDRRC, N = 248 from 14 sires) and a cooperating breeding program (COOP, N = 186 from 34 sires). Polymorphisms were SNP mutations in intron 4 (C ... more

M.G. Thomas; R.M. Enns; K.L. Shirley; M.D. Garcia; A.J. Garrett; G.A. Silver
Dog breeds; Growth; Polymorphism; SNP; SOCS2

Suppressor of cytokine signaling 2 (SOCS2) is a negative regulator of growth hormone signaling. The deletion of SOCS2 in mice results in a 30-50% increase in post-natal growth. In an effort to identify polymorphisms in the SOCS2 gene that may be associated with body size in dogs, we characterized the canine SOCS2 gene and analyzed its genetic diversity among small and large dog breeds ... more

G. Rincón; A.E. Young; D.L. Bannasch; J.F. Medrano
Beef; Bovine; Genome; Livestock; Molecular markers; SNP

We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms ... more

L.F.B. Pinto; J.B.S. Ferraz; V.B. Pedrosa; J.P. Eler; F.V. Meirelles; M.N. Bonin; F.M. Rezende; M.E. Carvalho; D.C. Cucco; R.C.G. Silva
IL-6; Insulin resistance; SNP; TCF7L2; TNF; VDR

A population-based study was undertaken to evaluate linkage between single-nucleotide polymorphisms known as risk factors and type 2 diabetes in an Indian population. The study population was comprised of 40 normal glucose-tolerant individuals (21 males and 19 females) and 40 type 2 diabetes patients (21 males and 19 females). The genes and their corresponding single-nucleotide ... more

P.N. Mukhopadhyaya; A. Acharya; Y. Chavan; S.S. Purohit; A. Mutha