Research Article

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08/16/2011
IGF-1; PCR-SSCP; SNP

The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were observed, among which, one single nucleotide polymorphism, ... more

L.L. Hao; H. Yu; Y. Zhang; S.C. Sun; S.C. Liu; Y.Z. Zeng; Y.X. Ai; H.Z. Jiang
11/09/2010
Body measurement traits; Goat; LF gene; Milk composition; PCR-SSCP; SNP

Milk composition and body measurement traits, influenced by genes and environmental factors, play important roles in value assessments of efficiency and productivity in dairy goats. Lactoferrin (LF), involved in the efficient expression of protein in milk, is also an anabolic factor in skeletal tissue and a potent osteoblast survival factor. Therefore, it is an important candidate gene ... more

B.L. Guo; Y. Jiao; C. He; L.X. Wei; Z.H. Chang; X.P. Yue; X.Y. Lan; H. Chen; C.Z. Lei
09/30/2004
Carcass traits; Cattle; Heritability; Slaughter end points

We present estimates of heritability for carcass traits of cattle published in the scientific literature. Seventy-two papers published from 1962 to 2004, which reported estimates of heritability for carcass traits, were reviewed. The unweighted means of estimates of heritability for 14 carcass traits by slaughter end point (age, weight, and fat depth) were calculated. Among the three end ... more

A.Ríos Utrera; L.Dale Van Vleck
05/31/2006
Blood coagulation; Carrier DNA sample; Cattle; Factor XI; Recessive DNA sample

An insertion mutation within exon 12 of the factor XI gene has been described in Holstein cattle. This has opened the prospect for large-scale screening of cattle using the polymerase chain reaction (PCR) technique for the rapid identification of heterozygous animals. To facilitate such a screening process, the mutant and normal alleles of factor XI gene, represented by 244- and 320-bp ... more

P.N. Mukhopadhyaya; M. Jha; P. Muraleedharan; R.R. Gupta; R.N. Rathod; H.H. Mehta; V.K. Khoda
11/24/2009
Animal reproduction; Assisted reproduction technologies; Cattle

Embryos produced by hormonal superstimulation have been used as an in vivo control in most published research on embryo gene expression. However, it is not known if this is the most appropriate control for gene expression profile studies. We compared the expression of GRB-10, IGF-II, IGF-IIR, MnSOD, GPX-4, catalase, BAX ... more

T.C.D. Mundim; A.F. Ramos; R. Sartori; M.A.N. Dode; E.O. Melo; L.F.S. Gomes; R. Rumpf; M.M. Franco
08/25/2011
Association analysis; Body measurement; Cattle; CDK6 gene; SNP polymorphism

Cyclin-dependent kinase 6 (CDK6) is a key element of D-type cyclin holoenzymes. It is involved in the regulation of the G1-phase of the cell cycle and is considered to be an important candidate gene for selection of body measurement traits through marker-assisted selection. We cloned the promoter sequence of this gene in bovines and found it to share high similarity with that of the human ... more

Y.F. Liu; L.S. Zan; W.T. Cui; Y.P. Xin; Y. Jiao; K. Li
08/21/2013
blood; BPV; Cattle; Viral gene expression

Papillomaviruses (PV) are double-stranded DNA viruses that can cause benignant and malignant tumors in amniotes. There are 13 types of bovine papillomavirus (BPV-1 to -13); they have been found in reproductive tissues and body fluids. Normally these viruses are detected in epithelial tissue. We looked for BPV in the blood of healthy cattle and cattle with papillomatosis, using PCR and RT- ... more

M.A.R. Silva; B.M.F. De Albuquerque; N.E. Pontes; L.C.A. Coutinho; M.C.G. Leitão; M.C. Reis; R.S. Castro; A.C. Freitas
03/22/2012
Association; Cattle; PRDM16 gene; Sequence variants

As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms in exons 2, 3, 4, 5, 7, 8, and 9 of the PRDM16 gene ... more

J. Wang; C. Wang; R. Tian; Y.Z. Huang; X.S. Lai; X.Y. Lan; J.Q. Wang; H. Chen
01/23/2015
Alternative splicing; CAPN3; Cattle; Transcript

Calpain-3 (CAPN3) is a member of the calpain family of Ca2+-regulated cysteine proteases, which play an important role in sarcomere remodeling and mitochondrial protein turnover, and thus, regulating beef tenderness in cattle. Currently, multiple CAPN3 transcripts have been detected in human, monkey, rat, and rabbit. However, whether this transcript is present in cattle remains ... more

S.Y. Liu; H. Jiang; B. Yuan; Y. Gao; L.S. Dai; J.B. Zhang
09/16/2011
Bovine C/EBPa gene; Genetic polymorphism; Meat quality traits; PCR-SSCP

CCAAT/enhancer-binding protein alpha (C/EBPa) is an essential transcriptional factor regulating the differentiation of adipocytes. We report a novel single nucleotide polymorphism (C271A) of the C/EBPa gene in six indigenous Chinese cattle breeds using PCR-SSCP and DNA sequencing methods. Allele frequencies were investigated and evaluated by the χ2 test in 817 individuals; all ... more

H. Wang; L.S. Zan; H.B. Wang; F.B. Song

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