Research Article

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10/25/2011
Bovine; Cattle; Embryo; Sexing; Single cell; TSPY gene

The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single ... more

M.C.A. Carneiro; P.L. Takeuchi; A. Araújo; R.B. Lôbo; F.P. Elias; R.A. Vila; C.L. Miranda-Furtado; E.S. Ramos
12/12/2011
Association; Cattle; Genotypic frequencies; meat quality; Mutation; Myogenic factor 5

Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live ... more

J.A. Ujan; L.S. Zan; S.A. Ujan; C. Adoligbe; H.B. Wang
10/27/2011
BLV testing; Bovine leukemia virus; Cattle; Leukosis; Native Iranian and Russian breeds; PCR

Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

M.R. Mohammadabadi; M. Soflaei; H. Mostafavi; M. Honarmand
11/04/2011
Body measurement traits; Cattle; MyoG; Polymorphisms

Using PCR-SSCP and DNA sequencing technology, we examined the association of single nucleotide polymorphisms (SNPs) in the bovine MyoG gene with body measurement traits in 779 individuals of six native Chinese cattle breeds, namely Luxi, Luxi × Simmental crossbred, Nanyang, Xia’nan, Jiaxian red, and Qinchuan. A novel SNP, T314C, was detected. Allelic frequencies of MyoG- ... more

M. Xue; L.S. Zan; L. Gao; H.B. Wang
09/21/2011
Cattle; CYP21; Genotype-phenotype association; IGF2; SNPs

We analyzed two single nucleotide polymorphisms (SNPs) of the IGF2 and CYP21 genes in Nellore cattle participating in the Brazilian Animal Breeding Program. The SNPs were found in exon 6 of the IGF2 (insulin-like growth factor 2) gene (RFLP/MboII) as well as in the promoter region of the CYP21 (steroid 21-hydroxylase) gene (RFLP/HpaII) ... more

M. da Silva; A.F.L. Rios; E.S. Ramos; R.B. Lôbo; H.N. Oliveira; M.A.R. de Freitas
07/12/2011
Bov-A2; Cattle; CYP21 gene; Epigenetics; Methylation; SINE

The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu ... more

A.M. da Silva; M.A.R. de Freitas; A.F.L. Rios; A. Renzi; R.B. Lôbo; M.A.V. Galerani; R.A. Vila; E.S. Ramos
06/21/2011
Association study; Carcass traits; Cattle; Microsatellite markers

Investigation of molecular marker effects on production traits is essential to define marker assisted selection strategies in beef cattle. We looked for a possible association of molecular markers and backfat thickness (BFT) and rib eye area (REA) in Canchim (5/8 Charolais + 3/8 Zebu) and MA (offspring of Charolais bulls and 1/2 Canchim + 1/2 Zebu cows) animals raised exclusively on ... more

S.L. Meirelles; G.V. Gouveia; G. Gasparin; M.M. Alencar; J.J.S. Gouveia; L.C.A. Regitano
05/31/2011
Body measurement traits; Cattle; GDF10 gene; SNP

Body measurement traits are known to play numerous important roles in the assessment of productivity and economic value. They are influenced by several factors, among which genetic factors are predominant. The gene GDF10 is involved in skeletal morphogenesis and is associated with body measurement traits. It may be an important candidate gene for marker-assisted selection. We ... more

C. Adoligbe; L.S. Zan; H.B. Wang; J.A. Ujjan
05/11/2010
BMP4 gene; Body measurement traits; Cattle; Microsatellite

We examined the variation of the BMP4 gene in four Chinese indigenous cattle breeds and investigated the association of this polymorphism with body measurement traits. Using PCR-SSCP and DNA sequencing, a polymorphic microsatellite was detected in the third exon of the bovine BMP4 gene in 459 samples from four Chinese indigenous cattle breeds, Qinchuan, Luxi, Nanyang, ... more

X. Zhong; L.S. Zan; H.B. Wang; Y.F. Liu
02/18/2010
Cattle; Cell culture confluence; Cell cycle; Embryo aggregation; Somatic cell nuclear transfer

The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bisected and selected by DNA staining. One or ... more

R.P.C. Gerger; E.S. Ribeiro; F. Forell; L.R. Bertolini; J.L. Rodrigues; C.E. Ambrósio; M.A. Miglino; A. Mezzalira; M. Bertolini

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