Research Article

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Goat; Lectin; Seminal plasma; Spermadhesin

Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we ... more

D.Ítalo Alv Teixeira; L.Magalhães Melo; C.Alberto de Gadelha; R.Maranguape da Cunha; C. Bloch; G. Rádis-Baptista; B.Sousa Cavada; V.José de F. Freitas
10/05/2016
Genetic diversity; Microsatellite; Pinctada fucata; Polymorphism

The pearl oyster Pinctada fucata is a commercially important marine shellfish. As a result, genetic improvement and selective-breeding program have been conducted for this species. Polymorphic microsatellites are effective molecular markers to investigate molecular marker-assisted selection and genetic variance. In this study, microsatellite DNAs were screened and characterized ... more

S.G. Fan; J.X. Wang; D.L. Zhang; Y.H. Guo; D.H. Yu; S.G. Fan; J.X. Wang; D.L. Zhang; Y.H. Guo; D.H. Yu
11/03/2016
High-resolution melting; Pinctada fucata; SNP; Transcriptome sequencing

The pearl oyster Pinctada fucata is an important commercial marine shellfish that is cultured for producing saltwater pearls. In this study, 468 single nucleotide polymorphisms (SNPs) were screened from P. fucata transcriptome data, and 119 polymorphic SNPs were successfully isolated by a two-step small-amplicon high-resolution melting assay. Of ... more

S.G. Fan; J.F. Wei; Y.H. Guo; G.J. Huang; D.H. Yu; S.G. Fan; J.F. Wei; Y.H. Guo; G.J. Huang; D.H. Yu; S.G. Fan; J.F. Wei; Y.H. Guo; G.J. Huang; D.H. Yu
01/26/2015
Immune response; Pearl oyster; Pinctada martensii; Purple acid phosphatase

Purple acid phosphatases (PAPs), also known as type 5 acid phosphatases, are widely present in animals, plants, and fungi. In mammal, PAP was reported to participate in immune defense and bone resorption. In this study, the characteristics and potential functions of a PAP gene from pearl oyster Pinctada martensii (pm-PAP) were examined. The Pm-PAP cDNA was found to be 2777 base ... more

Q.H. Wang; Y. Jiao; X.D. Du; X.X. Zhao; R.L. Huang; Y.W. Deng; F. Yan
01/04/2011
Disease susceptibility; Innate immunity; Single-nucleotide polymorphisms; Toll-interleukin 1 receptor domain containing adaptor protein; Tuberculosis

Toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; also known as MAL) is an essential adaptor molecule in Toll-like receptor signaling, involved in activating the innate immune response during infection. Genetic variations in the TIRAP gene may influence human susceptibility to infectious disease. To date, in the Chinese population, a possible ... more

Y.X. Zhang; Y. Xue; M.Y. Zhao; H.J. Wang; J.C. Li; J.Y. Liu; F.J. Li; J.M. Zhou
12/19/2013
Adaptive immunity; Bovine; Flow cytometry; Innate immunity; Leukocyte

Differences in cellular and humoral immunity in Zebu (Bos taurus indicus) and European (B. taurus taurus) cattle breeds, which may be related to differences in resistance or susceptibility to infectious or parasitic diseases, are largely unknown. This study aimed to perform a comparative analysis of innate and adaptive immunity of European (including Holstein, Brown ... more

A.A. Macêdo; A.P.V. Marciano; L.M. Rocha; J.R.F. Alves-Júnior; A.M.C. Faria; J.F.F. Bittar; M.S.S. Araújo; R.L. Santos; O.A. Martins-Filho
02/08/2013
Innate immunity; Molecular evolution; PGLYRP1 gene; Positive selection; Primate; Purifying selection

Short-type peptidoglycan (PGN)-recognition protein 1 (PGLYRP1), an innate immunity protein that directly breaks down the structure of microbial cell wall PGNs, plays an important role both in antibacterial defenses and several inflammatory diseases. To explore the adaptive evolution of the PGLYRP1 gene in primates and provide insight into the function of this antibacterial ... more

W. Liu; Y.F. Yao; L. Zhou; Q.Y. Ni; H.L. Xu
04/29/2014
Collagenolytic serine protease; Eriocheir sinensis; Innate immunity

A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H, D, and S). Similarity analysis shows that Es-CLSP ... more

Q.Z. Yang; Z.J. Yang; Y. Zhang; X.L. Li; W. Zhang
06/10/2015
Atlantic salmon; Infections pancreatic necrosis virus; Innate immunity; TLR3; Toll-like receptor

Innate pathway activation is fundamental for early anti-viral defense in fish, but currently there is insufficient understanding of how salmonid fish identify viral molecules and activate these pathways. The Toll-like receptor (TLR) is believed to play a crucial role in host defense of pathogenic microbes in the innate immune system. In the present study, the full-length cDNA of Salmo ... more

R. Vidal; R. González; F. Gil
03/13/2015
Innate immunity; Macaca thibetana; Phylogenetic analysis; TLR4 gene; Toll-like receptor

In this study, the complete coding region sequence of an innate immune-related TLR4 gene was obtained from the Tibetan macaque (Macaca thibetana) genome via PCR and direct sequencing. The sequence had a total length of 2481 bp, contained 3 complete exons, and encoded 826 amino acids (AAs); its isoelectric point was 5.703, and the molecular weight was 94.72 kDa. The high ... more

Q.X. Dai; Y.F. Yao; Z.C. Qi; Y. Huang; Q.Y. Ni; M.W. Zhang; H.L. Xu