Research Article

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06/18/2015
Carbapenem; Carbapenemase; Efflux pumps; Porin; Pseudomonas aeruginosa; Resistance

We explored the mechanism of the development from sensitivity to resistance to carbapenem in Pseudomonas aeruginosa. Two P. aeruginosa strains were collected during treatment with carbapenem. Strain homology was investigated using pulsed-field gel electrophoresis. Porin oprD2 expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ... more

J.L. Shen; Y.P. Fang
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Clinical significance; E-cadherin; Epidermal growth factor receptor-tyrosine kinase inhibitor; Molecular treatment; Resistance

We examined the effect of E-cadherin expression on epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) molecular targeted therapy sensitivity/resistance. We treated MCF-7, MDA-MB-231, T24, SiHa, H460, SK-HEP-1, MHCC97-H, and THP-1 cells with the EGFR-TKIs PD153035 and gefitinib, and then tested the drug-resistance and sensitivity using the MTT method, calculated IC ... more

R.C. Xing; J. Zheng; W.H. Zheng; Z.P. Qin; W. Liu; R.C. Yao
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16S rRNA methylase gene; Acinetobacter baumannii; Aminoglycoside-modifying enzyme gene; Aminoglycosides; Molecular identification; Resistance

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T. Lin; C.G. Tang; Q.H. Li; J. Ji; H.Y. Ge; X.Y. Zhang; H.P. Sun
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alpha-Defensins; Animals; Gene expression; Gene Transfer Techniques; Lactuca; Plants, Genetically Modified; Rabbits

Lettuce (Lactuca sativa L.) is an annual plant of the daisy family, Asteraceae, with high food and medicinal value. However, the crop is susceptible to several viruses that are transmitted by aphids and is highly vulnerable to post-harvest diseases, as well as insect and mammal pests and fungal and bacterial diseases. Here, the rabbit defensin gene NP-1 was transferred into lettuce by ... more

D. Song; X. Xiong; W.F. Tu; W. Yao; H.W. Liang; F.J. Chen; Z.Q. He
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DNA movement; Manihot fortalezensis; Meloidogyne javanica; Resistance

Screening cassava germplasm for resistance to the root-knot nematode (Meloidogyne javanica) showed that periclinal chimera, which came from combination of the wild species Manihot fortalezensis and indigenous cultivar UnB 201, resulted in resistance to M. javanica. Apparently periclinal chimera acquired resistance from ... more

D.S. Ferreira; J.E. Cares; N.M.A. Nassar
09/02/2004
Cysteine proteinase; Expression; Heterodera glycines; Inhibitory activity; Nematode; Pro domain peptide

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active ... more

F.B. Silva; J.A.N. Batista; B.M. Marra; R.R. Fragoso; A.Carolina S. Monteiro; E.L.Z. Figueira; M.Fátima Grossi-de-Sá

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