Research Article

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Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by ... more

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Cattle; Growth traits; PCR-SSCP; SNPs; SST gene

Somatostatins play a crucial role in the regulation of growth and development in vertebrates, especially muscle growth. We assessed the association of somatostatin gene polymorphisms with growth traits by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and DNA sequencing methods in 694 individuals from six Chinese cattle breeds. A novel single nucleotide ... more

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BoLA; Bovine; Candidate genes; Cattle; Molecular markers; Tick resistance

Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to many chemicals. More recently, vaccines have been used in ... more

M.L. Martinez; M.A. Machado; C.S. Nascimento; M.V.G.B. Silva; R.L. Teodoro; J. Furlong; M.C.A. Prata; A.L. Campos; M.F.M. Guimarães; A.L.S. Azevedo; M.F.A. Pires; R.S. Verneque
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Candidate genes; Cattle; DGAT1; PCR-RFLP; Zebu

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection ... more

G.A. Lacorte; M.A. Machado; M.L. Martinez; A.L. Campos; R.P. Maciel; R.S. Verneque; R.L. Teodoro; M.G.C.D. Peixoto; M.R.S. Carvalho; C.G. Fonseca
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Cattle; Cell isolation; Multiplex-PCR; Nuclear transfer; Transgene detection

Transgenesis in cattle has provided numerous opportunities for livestock production. The development of nuclear transfer (NT) technology has improved the production of transgenic livestock. However, the isolation of pure colonies from a single transfection event remains laborious and can be a constraint in the production of transgenic livestock. We used 96-well cell culture plates to ... more

E.O. Melo; R.V. Sousa; L.T. Iguma; L.T. Iguma; M.M. Franco; E.L. Rech; E.L. Rech; R. Rumpf
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Cattle; Effective population size; Inbreeding; Pigs; Sheep; Software

Genetic variation provides a basis upon which populations can be genetically improved. Management of animal genetic resources in order to minimize loss of genetic diversity both within and across breeds has recently received attention at different levels, e.g., breed, national and international levels. A major need for sustainable improvement and conservation programs is accurate ... more

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Bovine; Cattle; Embryo; Sexing; Single cell; TSPY gene

The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single ... more

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Association; Cattle; Genotypic frequencies; meat quality; Mutation; Myogenic factor 5

Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live ... more

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Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is an exogenous, B lymphotropic retrovirus belonging to the Retroviridae family that induces persistent lymphocytosis in cattle and sheep. PCR has proven to be particularly suitable for investigating herds of cattle with a very low incidence of BLV infection and for clarifying doubtful serological results ... more

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