Research Article

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The current study was designed to evaluate lipid peroxidation (via malondialdehyde) levels, the superoxide dismutase (SOD) gene expression profile, and SOD enzyme activity in tomato plants (Lycopersicum esculentum L.) subjected to different time periods of cold stress (control, 2, 4, 6, 8, and 10 days). Results revealed that maximum lipid peroxidation occurred in ... more

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Escherichia coli F18; Pig; Real-time PCR; TLR4 gene

TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order ... more

J. Wang; Z.Y. Pan; X.R. Zheng; Z.C. Wu; X.M. Su; G.Q. Zhu; X.G. Huang; S.L. Wu; W.B. Bao
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Colon cancer; Expression; NPRL2; Real-time PCR; Tumor suppressor gene

Genetic and epigenetic factors affecting DNA methylation and gene expression are known to be involved in the development of colon cancer, but the full range of genetic alterations and many key genes involved in the pathogenesis of colon cancer remain to be identified. NPRL2 is a candidate tumor suppressor gene identified in the human chromosome 3p21.3 region. We evaluated the role of this ... more

B. Yogurtcu; I. Hatemi; I. Aydin; N. Buyru
09/25/2012
Malaria; Mitochondrial DNA; Plasmodium falciparum; Plasmodium vivax; Real-time PCR

The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probes. Since the first study published ... more

C.R.T. Souza; T.A.A. Carvalho; R.C.G. Amaral; L.S. Cunha; M.G. Cunha; J.F. Guerreiro
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Sea Island cotton (Gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. Based on a transcript-derived fragment originated from transcriptome QTL mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cDNA, designated as GbPI4K, was first cloned, and its expression was characterized in the secondary ... more

H.W. Liu; R.F. Shi; X.F. Wang; Y.X. Pan; G.Y. Zang; Z.Y. Ma
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Bovine tuberculosis; Mycobacterium bovis; Real-time PCR; Validation

This study aimed to develop and validate real-time PCR for the diagnosis of Mycobacterium bovis isolates. Two hundred and seventy-four M. bovis isolates and 156 M. tuberculosis isolates were tested. Both qPCRs amplified all of the 274 M. bovis samples, but none of the 156 M. tuberculosis samples. The qPCR for PE-PGRS 20 had 91% efficiency and a ... more

M.L. Sales; A.A. Fonseca; L. Orzil; A.P. Alencar; M.A. Hodon; M.A. Issa; P.M.Soares Filho; M.R. Silva; A.P. Lage; M.B. Heinemann
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Germination; Real-time PCR; Seed quality; Seed vigor

Phytohormones have different characteristics and functions, and they may be subject to changes in their gene expression and synthesis during seed development. In this study, we evaluated the physiological qualities of habanero peppers (Capsicum chinense Jacquin) during seed development and the expression of genes involved in germination. Seeds were obtained from fruits harvested ... more

H.O. Santos; E.V.R. Von Pinho; I.V. Von Pinho; S.M.F. Dutra; T. Andrade; R.M. Guimarães
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Bacillus pumilus; Extracellular protease; Real-time PCR; Salt stress; Transcription

Bacillus pumilus is able to secrete subtilisin-like prote­ases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular pro­teases in B. pumilus (BA06) under salt stress were investigated using various methods, including a ... more

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Heading date; miRNA; Prediction model; Real-time PCR; Rice

Rice (Oryza sativa L.) is one of the most important food crops in the world. In Taiwan, due to the warm climate, there are two harvests annually. However, the yield and quality of rice can vary between each crop season in any given year. Previous reports have shown that microRNAs (miRNAs) play a crucial role in many developmental and physiological processes in plants. In this ... more

Y.C. Chen; W.S. Lin; R.K. Chen; Y.Y. Chao; S.W. Chin; F.C. Chen; C.Y. Lee

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